Robust Min‐system oscillation in the presence of internal photosynthetic membranes in cyanobacteria
Robust Min‐system oscillation in the presence of internal photosynthetic membranes in cyanobacteria
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DOI：
10.1111/mmi.13571
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发表时间：
2017-02
影响因子：
3.6
通讯作者：
Joshua S MacCready;Jory Schossau;K. Osteryoung;Daniel C. Ducat
中科院分区：
文献类型：
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作者：
Joshua S MacCready;Jory Schossau;K. Osteryoung;Daniel C. Ducat
大肠杆菌的振荡Min系统通过调节FtsZ环形成的位点来定义细胞分裂平面，并且代表了自然界中涌现的蛋白质自组织的最佳理解示例之一。Min系统蛋白质MinC、MinD和MinE（MinCDE）的振荡模式强烈依赖于它们结合的膜的几何形状。蓝藻内复杂的内部膜可以通过空间上封闭或隔离MinCDE与质膜来破坏这种自组织。在这里，它表明，在蓝细菌细长聚球藻PCC 7942中的Min系统从极点到极点振荡，尽管其广泛的类囊体网络施加了潜在的空间限制。此外，反应扩散模拟预测了在模拟的蓝藻细胞中的鲁棒振荡，前提是保持类囊体网络的渗透性以促进扩散，并表明Min蛋白需要对质膜的优先亲和力超过类囊体以正确定位FtsZ环。有趣的是，除了振荡之外，MinC还表现出依赖于MinD和DivIVA样蛋白Cdv3的中细胞定位，表明两个不同的MinC库在S中协调。细长的我们的结果首次提供了E外Min振荡的直接证据。大肠杆菌，并具有更广泛的意义，在细菌和细胞器与内部膜系统的Min‐system功能。
The oscillatory Min system of Escherichia coli defines the cell division plane by regulating the site of FtsZ‐ring formation and represents one of the best‐understood examples of emergent protein self‐organization in nature. The oscillatory patterns of the Min‐system proteins MinC, MinD and MinE (MinCDE) are strongly dependent on the geometry of membranes they bind. Complex internal membranes within cyanobacteria could disrupt this self‐organization by sterically occluding or sequestering MinCDE from the plasma membrane. Here, it was shown that the Min system in the cyanobacterium Synechococcus elongatus PCC 7942 oscillates from pole‐to‐pole despite the potential spatial constraints imposed by their extensive thylakoid network. Moreover, reaction‐diffusion simulations predict robust oscillations in modeled cyanobacterial cells provided that thylakoid network permeability is maintained to facilitate diffusion, and suggest that Min proteins require preferential affinity for the plasma membrane over thylakoids to correctly position the FtsZ ring. Interestingly, in addition to oscillating, MinC exhibits a midcell localization dependent on MinD and the DivIVA‐like protein Cdv3, indicating that two distinct pools of MinC are coordinated in S. elongatus. Our results provide the first direct evidence for Min oscillation outside of E. coli and have broader implications for Min‐system function in bacteria and organelles with internal membrane systems.