Expression of Inflammatory and Regenerative Genes in a Model of Liver Ischemia/Reperfusion and Partial Hepatectomy
Expression of Inflammatory and Regenerative Genes in a Model of Liver Ischemia/Reperfusion and Partial Hepatectomy
复制标题
DOI：
10.3109/08941939.2015.1060280
复制
发表时间：
2016-03-03
影响因子：
1.9
通讯作者：
Arkadopoulos, Nikolaos
中科院分区：
文献类型：
--
作者：
Athanasopoulos, Panagiotis;Mastoraki, Aikaterini;Arkadopoulos, Nikolaos
目的/目的：肝缺血/再灌注（I/R）描述了再灌注引起额外组织损伤的悖论。本研究的目的是研究猪I/R和延长肝切除术模型中发挥炎症和再生反应的基因mRNA表达。材料与方法：选用12头平均体重30 ~ 35kg的猪，随机分为I/R组8头和假手术组4头。I/R组行门静脉吻合和Pringle手法后行肝切除术。肝十二指肠韧带闭塞150min，残肝再灌注24h。在整个手术过程中稳定地接受血液样本，并进行肝脏活检以进行病理评估。再灌注开始后24h处死动物。结果：两组再灌注前期、后期血清AST、ALT、ALP、总胆红素值比较，差异均有统计学意义。I/R组iNOS、IL-1b、TGF-a mRNA表达无明显升高。相反，IL-6、STAT-3和E-selectin的mRNA修饰在I/R动物中表达显著增加。结论：在本次调查中，提出了一个新的猪I/R模型，并分析了具体参数，揭示了研究组之间的差异。
Purpose/Aim: Hepatic ischemia/reperfusion (I/R) describes the paradox of additional tissue injury caused by reperfusion. The aim of this survey was to investigate the mRNA expression of genes exerting their inflammatory and regenerative reaction in a porcine model of I/R and extended hepatectomy.Material and methods: Twelve pigs were used, weighing 30-35kg in average, which were allocated in two groups: the I/R group with eight pigs and the sham-operated (control) one with four pigs. The I/R group underwent portacaval anastomosis and Pringle maneuver followed by extended hepatectomy. The hepatoduodenal ligament was occluded for 150min and the liver remnant was reperfused for 24hours. Blood samples were steadily received throughout the surgical procedure, where hepatic biopsies were taken for pathological evaluation. Animals were sacrificed in 24hours after the onset of reperfusion.Results: Between the two groups, statistically significant differences were noticed in serum values of AST, ALT, ALP, and total bilirubin in the early and late phase of reperfusion. The mRNA expression of iNOS, IL-1b, and TGF-a did not increase significantly in the I/R group. Conversely, the mRNA modification of IL-6, STAT-3, and E-selectin demonstrated significantly increased expression in I/R animals.Conclusions: In the present survey, a new I/R swine model was proposed and specific parameters were analyzed, revealing differences between the study groups.