Determination and distribution of a female-specific protein in the brown planthopper, Nilaparvata lugens Stal (Homoptera: Delphacidae)
Determination and distribution of a female-specific protein in the brown planthopper, Nilaparvata lugens Stal (Homoptera: Delphacidae)
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DOI：
10.1016/j.tice.2004.09.003
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发表时间：
2005-02-01
期刊：
影响因子：
2.6
通讯作者：
Hou, RE
中科院分区：
文献类型：
--
作者：
Cheng, DJ;Hou, RE
褐飞虱（Nilaparvata lugens Stal）雌性、雄性和5龄若虫的血淋巴和体或卵巢均质液的电泳分析显示，雌性的蛋白带为175 kDa。免疫印迹试验显示，该蛋白与来自女性身体或卵巢匀浆的175 kDa蛋白有阳性反应。血淋巴中的这种蛋白很可能是卵黄蛋白原（Vg）。通过免疫荧光和免疫金标记技术测定Vg的分布。结果表明，卵黄颗粒、卵泡细胞间隙、血淋巴和卵巢上皮塞均有阳性免疫荧光反应。此外，成虫和各种若虫菌丝细胞中的酵母样共生体（YLS）以及游离于血淋巴或进入卵母细胞的酵母样共生体（YLS）也表现出阳性反应。电镜图显示，免疫金颗粒主要分布在蛋黄团和其他组织的YLS中。特别是不同发育阶段的YLS都含有免疫金颗粒，这表明共生体与雌性特异性蛋白的产生有一定的关系。(C) 2004 Elsevier Ltd.版权所有。
Electrophoretic analyses of hemolymph and body or ovary homogenates from reproducing females, males, and 5th instar nymphs of the brown planthopper, Nilaparvata lugens Stal, revealed a protein band of 175 kDa in females. An immunoblot test using antibody against this protein showed a positive reaction with a 175 kDa protein from female body or ovary homogenates. It is likely that this protein in hemolymph is vitellogenin (Vg). Distribution of Vg was determined by immunofluorescence and immunogold labeling techniques. The results showed that the positive immunofluorescence reactions were present in yolk particles, the intercellular space of follicle cells, hemolymph, and the epithelial plug of ovarioles. In addition, the yeast-like symbiotes (YLS) in mycetocytes of adults and various nymphal instars as well as those free in hemolymph or entering oocytes also exhibited a positive reaction. Electron micrographs showed that immunogold particles were found most in yolk mass and YLS over other tissues. Especially the YLS in various developmental stages all contained immunogold particles, implying that the symbiote is somewhat related with production of the female-specific protein. (C) 2004 Elsevier Ltd. All rights reserved.