Molecular characterization of the testis specific c‐abl mRNA in mouse.
Molecular characterization of the testis specific c‐abl mRNA in mouse.
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小鼠睾丸特异性 c-abl mRNA 的分子特征。
DOI：
10.1002/j.1460-2075.1987.tb02749.x
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Gerard Grosveld
中科院分区：
文献类型：
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作者：
Dies Meijer;A. Hermans;M. Lindern;Ton;van Agthoven;A. D. Klein;Petra Mackenbachl;Anton;'. Grootegoed;Daniela Talarico;Giuliano Della;Valle;Gerard Grosveld
c-abl 基因编码蛋白酪氨酸激酶，并从至少两个启动子转录，产生长度约为 5 和 6 kb 的两种大小类别的转录物。这些 mRNA 仅在最 5' 外显子上有所不同，并编码大小相似但 N 末端不同的蛋白质。在小鼠睾丸中检测到额外丰富的 4 kb c-abl mRNA。该 mRNA 在成年小鼠的单倍体雄性生殖细胞中表达。在这里，我们描述了代表睾丸特异性 c-abl 转录物的 cDNA 的克隆和分子特征。我们发现 4 kb c-abl mRNA 是由与 5 kb mRNA 相同的启动子转录的 RNA 的选择性多腺苷酸化产生的。在这个较短的转录本中，聚腺苷酸化位点是不寻常的，因为它前面没有高度保守的六核苷酸 AAUAAA。该多腺苷酸化位点的使用去除了体细胞 c-abl mRNA 中存在的 1.2 kb 3' 序列，但不影响转录本的主要开放阅读框。对整个睾丸切片进行原位杂交表明，4 kb c-abl mRNA 在伸长的精子细胞中最为丰富。
The c‐abl gene encodes a protein tyrosine kinase and is transcribed from at least two promoters giving rise to transcripts of two size classes of approximately 5 and 6 kb in length. These mRNAs only differ in their most 5′ exon and encode proteins of similar size but with different N‐termini. In the mouse testis an additional abundant c‐abl mRNA of 4 kb is detected. This mRNA was shown to be expressed in the haploid male germ cells of the adult mouse. Here we describe the cloning and molecular characterization of a cDNA representing the testis specific c‐abl transcript. We show that the 4 kb c‐abl mRNA arises from alternative polyadenylation of an RNA transcribed from the same promoter as the 5 kb mRNA. The site of polyadenylation is unusual in this shorter transcript as it is not preceded by the highly conserved hexanucleotide AAUAAA. The use of this polyadenylation site removes 1.2 kb of 3′ sequences present in the somatic c‐abl mRNAs, but does not affect the main open reading frame of the transcript. Using in situ hybridization on whole testis sections it is shown that the 4 kb c‐abl mRNA is most abundant in the elongating spermatids.