Genes expressed in the Drosophila head reveal a role for fat cells in sex-specific physiology
Genes expressed in the Drosophila head reveal a role for fat cells in sex-specific physiology
复制标题
DOI：
10.1093/emboj/cdf556
复制
发表时间：
2002-10-15
期刊：
影响因子：
11.4
通讯作者：
Amrein, H
中科院分区：
文献类型：
--
作者：
Fujii, S;Amrein, H
果蝇性别决定级联的下游效应子大多是未知的，被认为介导了性分化、生理和行为的所有方面。在这里，我们采用基因表达系列分析（SAGE）来鉴定在头部表达的雄性和雌性效应子，并报告了46个性别偏向基因（>4倍/P < 0.01）。我们鉴定了四种新的男性或女性特异性基因，发现它们主要在头部的脂肪细胞中表达。Tsx（开启性别特异性）、sxe 1和sxe 2（性别特异性酶112）在雄性中表达，但在雌性中不表达，并且依赖于已知的性别决定途径，特别是Transformer（tra）及其下游靶向double-exX（dsx）。雌性特异性表达的第四个基因，fit（雌性特异性独立于Transformer），不受tra和dsx的控制，这表明了一些效应基因调控的替代途径。我们的研究结果表明，头部的脂肪细胞表达性别特异性效应子，从而在男性和女性头部产生不同的生理条件。我们认为，这些差异对男性和女性的大脑调节性别特异性的神经元过程的后果。
The downstream effectors of the Drosophila sex determination cascade are mostly unknown and thought to mediate all aspects of sexual differentiation, physiology and behavior. Here, we employed serial analysis of gene expression (SAGE) to identify male and female effectors expressed in the head, and report 46 sex-biased genes (>4-fold/P < 0.01). We characterized four novel, male- or female-specific genes and found that all are expressed mainly in the fat cells in the head. Tsx (turn on sex-specificity), sxe1 and sxe2 (sex-specific enzyme 112) are expressed in males, but not females, and are dependent on the known sex determination pathway, specifically transformer (tra) and its downstream target doublesex (dsx). Female-specific expression of the fourth gene,fit (female-specific independent of transformer), is not controlled by tra and dsx, suggesting an alternative pathway for the regulation of some effector genes. Our results indicate that fat cells in the head express sex-specific effectors, thereby generating distinct physiological conditions in the male and female head. We suggest that these differences have consequences on the male and female brain by modulating sex-specific neuronal processes.