STUDY OF CHANGES IN PROTEIN COMPOSITION OF MOUSE BRAIN DURING ONTOGENETIC DEVELOPMENT
STUDY OF CHANGES IN PROTEIN COMPOSITION OF MOUSE BRAIN DURING ONTOGENETIC DEVELOPMENT
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DOI：
10.1111/j.1471-4159.1971.tb00183.x
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发表时间：
1971-01-01
影响因子：
4.7
通讯作者：
SHOOTER, EM
中科院分区：
文献类型：
--
作者：
GROSSFELD, RM;SHOOTER, EM
成年小鼠大脑中的总蛋白质中，35%是水溶性的，65%是水不溶性的。使用水、Triton X - 100和十二烷基硫酸钠顺序处理的提取方案，有可能在不同的组中溶解至少90%的总量。采用孔隙梯度聚丙烯酰胺凝胶电泳对提取物进行定性分析。通过这种方法，每个部分被分解成20‐50个蛋白质条带。这种顺序提取-分离程序被用于研究小鼠脑蛋白的非遗传变化。最明显的蛋白质积累和蛋白质组成的改变发生在出生后的最初几周。在此之后，除了1%的SLS部分在小鼠的整个生命周期中继续增加外，定量和定性的变化都没有那么明显。尽管水溶性蛋白质在发育的早期阶段占主导地位，但洗涤剂可溶性成分随后占总蛋白质的比例稳步增加。
Of the total protein in an adult mouse brain, 35 per cent is water‐soluble and 65 per cent water‐insoluble. Using an extraction scheme of sequential treatment with water, Triton X‐100, and sodium lauryl sulphate, it was possible to solubilize at least 90 per cent of this total in distinct groups. Qualitative analysis of the extracts was achieved by electro‐phoresis in porosity gradient polyacrylamide gels. In this way, each fraction was resolved into 20‐50 protein bands.This sequential extraction‐fractionation procedure was employed in a study of the onto‐genetic changes in mouse brain proteins. The most pronounced accumulation of protein and alteration of protein composition occurred during the first few weeks after birth. Beyond that period, both quantitative and qualitative changes were much less dramatic, except in the 1 % SLS fraction, which continued to increase in size throughout the mouse's lifespan. Whereas the aqueous‐soluble proteins predominated during the very early stages of development, the detergent‐soluble constituents accounted subsequently for a steadily increasing proportion of the total protein.