Decoupling the coupling: surface attachment in actin-based motility.
Decoupling the coupling: surface attachment in actin-based motility.
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解耦耦合：基于肌动蛋白的运动中的表面附着。
DOI：
10.1021/cb700071d
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发表时间：
2007
影响因子：
4
通讯作者：
Theriot,JulieA
中科院分区：
文献类型：
--
作者：
Tsuchida,MarkA;Theriot,JulieA
肌动蛋白丝聚合为几种基于肌动蛋白的运动提供驱动力，推动负载，例如爬行细胞前缘的质膜、内体囊泡或细胞内细菌病原体。在这些系统中，分支的细丝网络不断生长，同时保持附着在负载上。以前的实验表明，在肌动蛋白丝成核和丝附着的蛋白质家族称为成核促进因子（NPFs），刺激肌动蛋白分支的形成和Arp 2/3复合物的成核的重要作用。最近的一份报告表明，N-WASP，NPF，使用不同的域来介导成核和附着在运动过程中。令人惊讶的细节的生化机制需要重新考虑的生物物理模型提出的肌动蛋白为基础的运动。
Actin filament polymerization provides the driving force for several kinds of actin-based motility, propelling loads such as the plasma membrane at the leading edge of a crawling cell, an endosomal vesicle, or an intracellular bacterial pathogen. In these systems, branched filament networks continuously grow while simultaneously remaining attached to the load. Previous experiments have suggested an important role in both actin filament nucleation and filament attachment for a family of proteins called nucleation-promoting factors (NPFs) that stimulate actin branch formation and nucleation by the Arp2/3 complex. A recent report demonstrates that N-WASP, an NPF, uses distinct domains to mediate nucleation and attachment during motility. The surprising details of the biochemical mechanism necessitate reconsideration of the biophysical models proposed for actin-based motility.