HIV-1 actively replicates in naive CD4+ T cells residing within human lymphoid tissues
HIV-1 actively replicates in naive CD4+ T cells residing within human lymphoid tissues
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DOI：
10.1016/s1074-7613(01)00217-5
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发表时间：
2001-10-01
期刊：
影响因子：
32.4
通讯作者：
Goldsmith, MA
中科院分区：
文献类型：
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作者：
Eckstein, DA;Penn, ML;Goldsmith, MA
尽管在体内的初始静息T细胞中检测到HIV-1基因表达，但这些细胞在体外对生产性感染具有抵抗力。然而我们发现，这是内生的。人类淋巴组织的微环境支持该人群的从头感染和耗竭。细胞周期分析和 DNA 标记实验证实这些细胞确实处于静止状态，因此被从头感染。对初始细胞内“爆发大小”的定量进一步证明这些细胞被有效感染并增加了局部病毒负荷。这些发现表明，淋巴组织支持静息的初始 T 细胞中活跃的 HIV-1 复制。此外，这些细胞不仅是潜伏病毒的储存库，而且是病毒复制的允许宿主，很可能将其作为消除的目标。
Although HIV-1 gene expression is detected in naive, resting T cells in vivo, such cells are resistant to productive infection in vitro. However, we found that the endogenous. microenvironment of human lymphoid tissues supports de novo infection and depletion of this population. Cell cycle analysis and DNA labeling experiments established that these cells were definitively quiescent and thus infected de novo. Quantitation of the "burst size" within naive cells further demonstrated that these cells were productively infected and contributed to the local viral burden. These findings demonstrate that lymphoid tissues support active HIV-1 replication in resting, naive T cells. Moreover, these cells are not solely reservoirs of latent virus but are permissive hosts for viral replication that likely targets them for elimination.