ALTERED REGULATION OF EPSTEIN-BARR VIRUS-INDUCED LYMPHOBLAST PROLIFERATION IN RHEUMATOID-ARTHRITIS LYMPHOID-CELLS
ALTERED REGULATION OF EPSTEIN-BARR VIRUS-INDUCED LYMPHOBLAST PROLIFERATION IN RHEUMATOID-ARTHRITIS LYMPHOID-CELLS
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DOI：
10.1002/art.1780230603
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发表时间：
1980-01-01
影响因子：
--
通讯作者：
SEEGMILLER, JE
中科院分区：
文献类型：
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作者：
BARDWICK, PA;BLUESTEIN, HG;SEEGMILLER, JE
B [bone marrow-derived] lymphoblast cell lines develop rapidly after in vitro infection with Epstein-Barr virus (EBV) in peripheral blood mononuclear cells (PBM) from patients with rheumatoid arthritis (RA). Cellular regulation of EBV-induced lymphoblast outgrowth in RA and normal lymphoid cell populations were compared. RA PBM developed proliferating B lymphoblast colonies in a median time of 10 days after exposure to an EBV-containing culture supernatant while normal PBM took 18 days to grow out under the same conditions. The more rapid outgrowth of RA cells was not due to therapy with anti-inflammatory or anti-rheumatic drugs. The time to outgrowth of the normal cells decreased to 11 days after T [thymus-derived] cell depletion. T cell depletion of the RA cells had less effect on the time to outgrowth but it was shortened to 8 days. B lymphoblast cell lines were established from purified T cell populations from 18 of 20 RA patients, while populations of T cells from normal donors with the same percentage of T and B cells did not produce lymphoblast cell lines when infected with EBV. The non-T lymphoid cells from 7 of 20 RA patients established permanent B lymphoblast cell lines spontaneously without EBV infection. Spontaneous outgrowth of lymphoid cells from normal donors occurred only once in 48 experiments. Abnormalities in cell-mediated regulation of EBV-induced cell proliferation were apparent in lymphoid cells from patients with RA.
