Histogenic Identification by RAPD Analysis of Leaves and Fruit of Newly Synthesized Chimeric Citrus
Histogenic Identification by RAPD Analysis of Leaves and Fruit of Newly Synthesized Chimeric Citrus
复制标题
新合成嵌合柑橘叶和果实的 RAPD 分析组织学鉴定
DOI：
10.21273/jashs.127.1.104
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发表时间：
2002
影响因子：
1.9
通讯作者：
M. Omura
中科院分区：
文献类型：
--
作者：
K. Sugawara;T. Wakizuka;A. Oowada;T. Moriguchi;M. Omura
利用随机扩增多态DNA（RAPD）技术对4个嫁接嵌合体、2个与哈姆林橙子（Citrus sinensis（L.）Osbeck）和'温州蜜柑'（C. unshiu Marc.），和两个自然发生的平周嵌合体品种，小林Mikan（C. unshiu和C. natsudaidai Hayata）和Kinkoji Unshu（C. unshiu和C. obovoidea hort.ex Takahashi）。叶片表皮和叶肉细胞的RAPD分析表明，这4个嫁接嵌合体为种间monekto嵌合体，其茎尖分生组织的最外层（组织发生层L-1）与内层（组织发生层L-2和L-3）不同。此外，由果皮内部细胞发育而成的汁囊是两个亲本来源品种细胞的混合物，是柑橘果实的主要可食部分。因此，囊泡至少由L-1和表皮下内层L-2细胞组成。这种种间嵌合体结构的确定（这是可能的分子技术）是一个有价值的发现，在改善柑橘通过故意使用周嵌合体。
Randomly amplified polymorphic DNA (RAPD) analysis was used to investigate the histogenic structure of leaf and fruit tissues in four graft chimeras, two intentional chimeras that were produced in combination with 'Hamlin' orange (Citrus sinensis (L.) Osbeck) and 'Satsuma' mandarin ( C. unshiu Marc.), and two naturally occurring periclinal chimera cultivars, Kobayashi Mikan (a graft chimera of C. unshiu and C. natsudaidai Hayata ), and Kinkoji Unshu (a graft chimera of C. unshiu and C. obovoidea hort. ex Takahashi). RAPD profiles of the lamina epidermis and the mesophyll cells of specific individuals indicated that the four graft chimeras were interspecific monekto chimeras, whose outermost layer (histogenic layer L-1) of the shoot apical meristem consisted of a species that was different from that in the inner layers (histogenic layers L-2 and L-3). Moreover, juice vesicles, which develop from the inside cells of the pericarp and become the main edible parts of Citrus fruit, were a mixture of the cells from both parental source cultivars. Therefore, the vesicles were at least composed of L-1 and subepidermal inner L-2 cells. This determination of interspecific chimeral construction (which was made possible by molecular techniques) is a valuable finding, in terms of improving Citrus through intentional use of periclinal chimerism.