Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells.
Hepatocytes and epithelial cells of donor origin in recipients of peripheral-blood stem cells.
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DOI：
10.1056/nejmoa3461002
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发表时间：
2002-03-07
影响因子：
158.5
通讯作者：
Estrov, Z
中科院分区：
文献类型：
--
作者：
Korbling, M;Katz, RL;Estrov, Z
背景：骨髓含有干细胞，具有分化为各种器官的成熟细胞的潜能。我们确定循环干细胞是否有类似的potential.Methods：从肝，胃肠道和皮肤活检标本，从12例谁经历了外周血（11例）或骨髓（1例）造血干细胞移植。六名女性患者接受了来自男性捐赠者的移植。其中5人接受了性别匹配的移植，1人接受了自体移植。通过细胞遗传学分析或限制性片段长度多态性分析验证造血干细胞植入。使用荧光原位杂交间期细胞核和免疫组化染色的细胞角蛋白，CD 45（白细胞共同抗原），和肝细胞特异性antigen.Results：所有6个收件人的性别不匹配的移植显示证据的供体来源的上皮细胞或肝细胞的存在下，活检进行了研究。XY阳性上皮细胞或肝细胞占活检标本组织切片中细胞的0 - 7%。这些细胞早在外周血干细胞移植后第13天就在肝组织中检测到，迟至第354天在皮肤组织中检测到。供体细胞在活检标本中的存在似乎并不依赖于由graft-versus-host diseases.Conclusions诱导的组织损伤的强度：循环干细胞可以分化成成熟的肝细胞和上皮细胞的皮肤和胃肠道。
Background: Bone marrow contains stem cells with the potential to differentiate into mature cells of various organs. We determined whether circulating stem cells have a similar potential.Methods: Biopsy specimens from the liver, gastrointestinal tract, and skin were obtained from 12 patients who had undergone transplantation of hematopoietic stem cells from peripheral blood (11 patients) or bone marrow (1 patient). Six female patients had received transplants from a male donor. Five had received a sex-matched transplant, and one had received an autologous transplant. Hematopoietic stem-cell engraftment was verified by cytogenetic analysis or restriction-fragment-length polymorphism analysis. The biopsies were studied for the presence of donor-derived epithelial cells or hepatocytes with the use of fluorescence in situ hybridization of interphase nuclei and immunohistochemical staining for cytokeratin, CD45 (leukocyte common antigen), and a hepatocyte-specific antigen.Results: All six recipients of sex-mismatched transplants showed evidence of complete hematopoietic donor chimerism. XY-positive epithelial cells or hepatocytes accounted for 0 to 7 percent of the cells in histologic sections of the biopsy specimens. These cells were detected in liver tissue as early as day 13 and in skin tissue as late as day 354 after the transplantation of peripheral-blood stem cells. The presence of donor cells in the biopsy specimens did not seem to depend on the intensity of tissue damage induced by graft-versus-host disease.Conclusions: Circulating stem cells can differentiate into mature hepatocytes and epithelial cells of the skin and gastrointestinal tract.