lncRNA LCPAT1 Upregulation Promotes Breast Cancer Progression via Enhancing MFAP2 Transcription
lncRNA LCPAT1 Upregulation Promotes Breast Cancer Progression via Enhancing MFAP2 Transcription
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DOI：
10.1016/j.omtn.2020.07.015
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发表时间：
2020-09-04
影响因子：
8.8
通讯作者：
Li, Dalin
中科院分区：
文献类型：
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作者：
Gong, Xue;Dong, Tuozhou;Li, Dalin
近年来，越来越多的证据支持长链非编码RNA（lncRNA）在肿瘤发生中的重要性。然而，将lncRNA功能与癌症进展联系起来的机制仍然知之甚少。lncRNA LCPAT 1在肺癌中起作用。然而，它如何在乳腺癌（BC）中起作用在很大程度上尚不清楚。在本研究中，我们发现LCPAT 1在BC组织和细胞系中高度表达。LCPAT1高表达预示BC患者生存率低。LCPAT 1在体外促进BC细胞增殖、迁移和侵袭，同时抑制凋亡。LCPAT1敲低抑制体内BC生长，反之亦然。LCPAT1与RBBP4相互作用并将其募集到MFAP2（微纤维相关蛋白2）启动子，然后激活MFAP2转录。MFAP 2的恢复挽救了BC细胞中LCPAT 1敲低的作用。总之，LCPAT 1通过募集RBBP 4启动MFAP 2转录来促进BC进展。
The importance of long noncoding RNA (lncRNA) in tumorigenesis has been supported by increasing evidence in recent years. However, the mechanism linking lncRNA function with cancer progression remains poorly understood. lncRNA LCPAT1 plays a role in lung cancer. However, how it works in breast cancer (BC) is largely unclear. In this study, we found that LCPAT1 was highly expressed in BC tissues and cell lines. High LCPAT1 expression predicted a low survival rate in BC patients. LCPAT1 promoted BC cell proliferation, migration, and invasion while inhibiting apoptosis in vitro. LCPAT1 knockdown suppressed BC growth in vivo and vice versa. LCPAT1 interacted with RBBP4 and recruited it to the MFAP2 (microfibril-associated protein 2) promoter and then activated MFAP2 transcription. Restoration of MFAP2 rescued the effects of LCPAT1 knockdown in BC cells. In sum, LCPAT1 promotes BC progression through recruiting RBBP4 to initiate MFAP2 transcription.