Cloning, expression, and protective immunity in mice of a gene encoding the diagnostic antigen P-29 of Echinococcus granulosus
Cloning, expression, and protective immunity in mice of a gene encoding the diagnostic antigen P-29 of Echinococcus granulosus
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编码细粒棘球绦虫诊断抗原 P-29 的基因在小鼠中的克隆、表达和保护性免疫
DOI：
10.1093/abbs/gmn009
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发表时间：
2009-01-01
影响因子：
3.7
通讯作者：
Zhao, Wei
中科院分区：
文献类型：
--
作者：
Shi, Zhiyun;Wang, Yana;Zhao, Wei
细粒棘球绦虫是引起棘球蚴病的重要病原体，是一种世界性分布的重要人兽共患病。本研究从大肠杆菌中克隆了诊断抗原P-29。并在大肠杆菌中表达。序列分析表明，EgP-29含有717 bp的开放阅读框，编码238个氨基酸，预测分子量为27.1 kDa。重组EgP-29（rEgP-29）能被小鼠抗血清识别。用酶联免疫吸附试验检测特异性抗体。用rEgP-29免疫小鼠，并腹腔内用E.免疫保护率达96.6%（P < 0.05）。因此，rEgP-29蛋白是预防继发性棘球蚴病的有效疫苗的有希望的候选者。
Taeniid tapeworm Echinococcus granulosus is the causative agent of Echinococcosis, an important zoonosis with worldwide distribution. In this study, a diagnostic antigen P-29 was cloned from E. granulosus and expressed in Escherichia coli. Sequence analysis showed that EgP-29 contains 717-bp open reading frame and encodes a protein of 238 amino acid residues with a predicted molecular weight of 27.1 kDa. The recombinant EgP-29 (rEgP-29) could be recognized with anti-mice sera in Western blotting. The specific antibody was detected by enzyme-linked immunosorbent assay. Mice vaccinated with rEgP-29 and challenged intraperitoneally with E. granulosus protoscoleces revealed significant protective immunity of 96.6% ( P < 0.05), compared with the control group. Thus, rEgP-29 protein is a promising candidate for an effective vaccine to prevent secondary echinococcosis.