EFFECTS OF NITROGEN SOURCES AND N/P RATION ON CELL GROWTH, TOTAL LIPID CONTENT AND FATTY ACID COMPOSITION OF Nannochloropsis oculata
EFFECTS OF NITROGEN SOURCES AND N/P RATION ON CELL GROWTH, TOTAL LIPID CONTENT AND FATTY ACID COMPOSITION OF Nannochloropsis oculata
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发表时间：
2000
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通讯作者：
W. Dong
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作者：
W. Dong
研究了3种氮源（NaNO 3、NH 4Cl和NH 4 NO3）和N/P对海洋微藻微拟球藻（Nannochloropsis oculata）细胞生长、总脂含量和脂肪酸组成的影响。结果表明，当N/P从10增加到90时，对细胞生长的影响不大，总脂含量维持在约10%。干重的20%。16∶0、16∶ 1 n9和20∶ 5 n3（EPA）为主要脂肪酸，占总脂肪酸的70%~ 80%.结果表明，在约20 ℃时，n3和PUFAs在总脂肪酸中的比例达到最大值，而在约20 ℃时，n3和PUFAs的比例达到最大值.在N/P20培养基中，产酶率分别为30%和35%。在三种氮源中，以氮源的生长速率和钙含量最高。在相对较低的N/P比54下，用NaNO 3获得EPA占总脂肪酸的30%。因此，NaNO 3是N.有眼的结果还表明，EPA含量可以增加到约10%。在添加4倍量外源营养盐的培养基中，其转化率为对照的35%。说明在富氮、富磷培养基中，细胞始终处于快速生长和EPA富集阶段，这也是该物种大量培养中EPA高产的关键步骤。
The effects of three nitrogen sources(NaNO3, NH4Cl and NH4NO3) and N/P on cell growth, total lipid content and fatty acid composition of marine microalgae Nannochloropsis oculata were investigated in this paper. Results show that when N/P increased from 10 to 90, a slightly effect on cell growth was observed, and total lipid content was maintained at ca. 20% of dry weight. 16∶0, 16∶1n 9 and 20∶5n 3 (EPA) were the dominant fatty acids, about 70% 80% of total fatty acids. The maximal ratio of 20∶5n 3 and PUFAs composition in total fatty acid were obtained at ca. 30% and 35% respectively in a medium of N/P20. Among the three nitrogen sources, a higher growth rate and ca. 30% of EPA to total fatty acid were obtained at a relatively lower N/P ratio at 54 with NaNO3. Thus, NaNO3 was the best nitrogen source for cell growth, synthesis and accumulation of EPA in N. oculata. It was also shown that EPA content could be increased to ca. 35% in medium with 4 times of exogenous nutrient salts to the control. It is indicated that cells were constantly at a rapid growth and EPA rich stage in culture medium rich in nitrogen and phosphate, and it was also the key step for the high yield of EPA in mass culture of this species.