Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera: Noctuidae)
Studies of sex pheromone production under neuroendocrine control by analytical and morphological means in the oriental armyworm, Pseudaletia separata, Walker (Lepidoptera: Noctuidae)
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DOI：
10.1016/j.ygcen.2011.02.018
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发表时间：
2011-05-15
影响因子：
2.7
通讯作者：
Matsumoto, Shogo
中科院分区：
文献类型：
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作者：
Adrien Fonagy;Moto, Ken'ichi;Matsumoto, Shogo
大多数雌蛾产生种特异性信息素混合物的修饰表皮信息素腺（PG）细胞通常位于第8和第9腹节之间。在脂肪酸从头合成过程中或之前，或在含氧官能团形成时，其生物合成通常受到信息素生物合成激活神经肽（PBAN）的调节。对粘虫（Pseudaletia separata）的生活史、鸣声、PG形态、信息素产生的日变化及其激素调节等方面的信息有限，我们在暗期每隔2 h测定一次信息素滴度（16：8; L：D）。在第2天（直到第4- 5天），在暗相的第6小时，共混比稳定，Z-11- 16 OH：16 OH：Z-11 - 16 Ac：16 Ac的比例分别为27.5：12.8：44.4：15.3。从这个时候开始，雌性动物表现出了呼唤行为。光镜和荧光显微镜观察发现，PG由第9腹节（B）背外侧区和节间膜（A部分）组成，约占气相色谱总产量的35%。比率没有显示差异。我们没有发现前体（三酰甘油）积累的形式脂滴，这意味着PBAN刺激从头生物合成的16：酰基前体。在体内，向3天大的16-18 h断头雌性动物中注射（1-3 x 5 pmol，2 h间隔）B-PBAN刺激A和B部分中的信息素产生。混合物分析，包括比例表明刺激的合成的初始阶段，但脂肪酰基中间体的去饱和不按比例遵循。更多的饱和脂肪酸从可用的池转化为最终的OH和Ac，相比，女性保持完整的暗期。体外研究（PG在0.25或0.5 μ M的β-PBAN存在下孵育4-6小时，特别是剩余的2 mM丙二酰-CoA）显示，与天然混合物或体内注射相比，饱和组分比高于不饱和组分。(C)2011 Elsevier Inc. All rights reserved.
Most female moths produce species-specific sex pheromone blends in the modified epidermal pheromone gland (PG) cells generally located between the 8 and 9th abdominal segments. The biosynthesis is often regulated by pheromone biosynthesis activating neuropeptide (PBAN) either in or prior to de novo fatty acid synthesis or at the formation of oxygenated functional group. In Pseudaletia separata, information about life span, calling, PG morphology, daily fluctuation of pheromone production and its hormonal regulation is limited.We measured pheromone titer daily (16:8; L:D) at 2 h intervals in scotophase. Blend ratio stabilized during the 2nd day (till 4-5th) at 6th hour of scotophase, with the ratio of 27.5:12.8:44.4:15.3 for Z-11-16OH:16OH:Z-11-16Ac:16Ac, respectively. Females showed calling behavior from this time. We found with light and fluorescence microscopy that PG consisted of intersegmental membrane (A part), and dorso-lateral region of 9th abdominal segment (B part), encountering for similar to 35% of total production revealed by gas chromatography. Ratios did not reveal difference. We did not find precursor (triacylglycerols) accumulation in form of lipid droplets, implying that PBAN stimulates de novo biosynthesis of 16:acyl precursors. In vivo Hez-PBAN injections (1-3 x 5 pmol, 2 h intervals) into 3 days old 16-18 h decapitated females stimulated pheromone production, both in A and B parts. Blend analyses including ratios suggest stimulation of the initial phase of synthesis, but desaturation of fatty acyl intermediates do not follow proportionally. More saturated fatty acid is converted from the available pool to the final OH and Ac, compared to females kept intact in scotophase. In vitro studies (PGs incubated 4-6 h in the presence of 0.25 or 0.5 mu M Hez-PBAN, especially with surplus 2 mM malonyl-CoA) revealed higher saturated component ratio than the unsaturated, compared to natural blend or in vivo injections. (C) 2011 Elsevier Inc. All rights reserved.