Preparation and verification of a monoclonal antibody against a conserved linear epitope in enterovirus A protein 2C
Preparation and verification of a monoclonal antibody against a conserved linear epitope in enterovirus A protein 2C
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DOI：
10.1016/j.jviromet.2021.114298
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发表时间：
2021-09-24
影响因子：
3.1
通讯作者：
Zhang, Shuye
中科院分区：
文献类型：
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作者：
Liu, Lizhen;Wang, Min;Zhang, Shuye
肠道病毒A（EV-A）种类是手足口病（HFMD）的主要病原体，手足口病是一个严重的公共卫生问题。缺乏合适的试剂阻碍了对这些病毒感染机制的研究。肠道病毒2C蛋白是肠道病毒的一种非结构蛋白，在病毒复制和抗病毒免疫中起重要作用。本文报道了用柯萨奇病毒A10蛋白2C（CVA 10 -2C）免疫小鼠制备单克隆抗体并进行检测。这种抗体可以识别大多数EV-A类型，包括常规和非常规群体。我们还绘制了抗体表位SLATGIIARA，它在EV-A物种中高度保守，位于ATP酶结构域。一些关键氨基酸包括G140、I141、I142和R144。本研究成功制备了抗多种伊娃的重组单克隆抗体，并证实了其特异性，为进一步研究肠道病毒A的感染及其在病原体诊断、抗病毒治疗等方面的应用奠定了基础。
Enterovirus A (EV-A) species are the main agents responsible for hand, foot, and mouth disease (HFMD), a serious public health concern. Lack of appropriate reagents prevents the mechanistic study of these virus in-fections. 2C protein, a non-structural protein of Enterovirus, is crucial for viral replication and antiviral im-munity. Here, preparation and testing of a monoclonal antibody by immunizing mice with Coxsackievirus A10 protein 2C (CVA10-2C) was reported. This antibody could identify most EV-A types, both conventional and unconventional groups. We also mapped the antibody epitope SLATGIIARA, which is highly conserved in EV-A species and located in the ATPase domain. Some key amino acids include G140, I141, I142, and R144. In conclusion, we generated a recombinant monoclonal antibody against multiple EVA types and confirmed its performance, which may facilitate the future study of Enterovirus A infection and many potential applications, such as the diagnosis of pathogen or the development of antiviral therapies.