Aging increases ischemia-reperfusion injury in the isolated, buffer-perfused heart.
Aging increases ischemia-reperfusion injury in the isolated, buffer-perfused heart.
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发表时间：
1994-12
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通讯作者：
E. Lesnefsky;D. Gallo;Jian-wen Ye;T. Whittingham;W. Lust
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作者：
E. Lesnefsky;D. Gallo;Jian-wen Ye;T. Whittingham;W. Lust
与整个成人人群相比，老年患者急性心肌梗死后的生存率降低。尽管一些心脏和非心脏原因可能导致死亡率增加，但关于缺血和再灌注期间衰老心肌对损伤的相对易感性知之甚少。我们假设老年人的心脏本质上比成年人的心脏更容易受到损害。将来自老年动物（Fischer 344大鼠，24月龄）的分离的缓冲液灌注大鼠心脏的恢复与从相同品系获得的成年心脏（6月龄）的恢复进行比较。心脏缺血25分钟，再灌注30分钟。与成人（n = 5）相比，老年人（n = 5）心脏的血流动力学恢复降低，包括发展的压力（缺血前基线的%：老年人31% +/- 4% vs成人57% +/-4%，p < 0.01）。老年心脏也持续更大的组织损伤，在30分钟的再灌注期间肌酸激酶的释放显著增加（老年人2950 +/- 500 U vs成人860 +/- 345 U，p < 0.01）。总蛋白和乳酸脱氢酶（肌细胞损伤的其他标志物）的释放也增加。因此，老年大鼠心脏比成年大鼠心脏更容易受到缺血再灌注损伤。在老年心肌实验模型中，缺血和再灌注期间的更大损伤提高了患有急性心肌梗死的老年患者中缺血性损伤更快速进展的可能性。
Survival after acute myocardial infarction is decreased in elderly patients as compared with the overall adult population. Although several cardiac and noncardiac causes could contribute to the increased mortality rate, little is known regarding the relative susceptibility of aging myocardium to injury during ischemia and reperfusion. We hypothesized that the elderly heart is intrinsically more susceptible to damage than the adult heart. The recovery of isolated, buffer-perfused rat hearts from elderly animals (Fischer 344 rats, 24 months of age) was compared with that of adult hearts (6 months of age) obtained from the same strain. Hearts underwent 25 minutes of ischemia followed by 30 minutes of reperfusion. Hemodynamic recovery was decreased in elderly (n = 5) as compared with adult (n = 5) hearts, including developed pressure (% of preischemic baseline: elderly 31% +/- 4% vs adult 57% +/- 4%, p < 0.01). Elderly hearts also sustained greater tissue damage, with a markedly increased release of creatine kinase (elderly 2950 +/- 500 U vs adult 860 +/- 345 U, p < 0.01) during the 30-minute reperfusion period. The release of total protein and lactate dehydrogenase, other markers of myocyte injury, was also increased. Thus the elderly rat heart is more susceptible than the adult rat heart to ischemia-reperfusion injury. Greater injury during ischemia and reperfusion in an experimental model of aged myocardium raises the possibility of a more rapid progression of ischemic damage in elderly patients suffering acute myocardial infarction.