Bone marrow mesenchymal stem cell-derived acellular matrix-coated chitosan/silk scaffolds for neural tissue regeneration
Bone marrow mesenchymal stem cell-derived acellular matrix-coated chitosan/silk scaffolds for neural tissue regeneration
复制标题
骨髓间充质干细胞来源的脱细胞基质包被的壳聚糖/丝支架用于神经组织再生
DOI：
10.1039/c6tb02959k
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发表时间：
2017
影响因子：
7
通讯作者：
Gu Xiaosong
中科院分区：
文献类型：
--
作者：
Xue Chengbin;Ren Hechun;Zhu Hui;Gu Xiaokun;Guo Qi;Zhou Yi;Huang Jing;Wang Shengran;Zha Guangbin;Gu Jianhui;Yang Yumin;Gu Yun;Gu Xiaosong
含有细胞外/脱细胞基质的神经支架是一种有前途的组织工程神经移植物（TENG）用于周围神经修复的设计。在这项研究中，我们通过培养狗骨髓间充质干细胞（BMSCs）到壳聚糖/丝素蛋白为基础的支架的表面，然后暴露细胞培养物脱细胞化的存款脱细胞基质（ACM）涂层的支架上的复合神经支架。这种天然生物材料为基础的，细胞衍生的ACM涂层神经支架，作为一种新型的神经移植物，用于桥接60 mm长的狗坐骨神经的神经间隙。在移植后12个月，行为，功能和组织学评价表明，我们开发的神经支架获得了令人满意的再生结果，这是非常接近自体神经移植物，周围神经修复公认的金标准。此外，通过用BMSC衍生的ACM修饰神经支架产生的额外治疗益处可能与ACM的独特神经活性相关，如通过ACM显著增强轴突再生和雪旺细胞增殖的体外实验发现所证明的。我们的研究结果将为含ACM的神经支架进入临床提供进一步的实验基础。
Extracellular/acellular matrix-containing neural scaffolds represent a promising design of a tissue engineered nerve graft (TENG) for peripheral nerve repair. In this study, we engineered a composite neural scaffold by culturing dog bone marrow mesenchymal stem cells (BMSCs) onto the surface of a chitosan/silk fibroin-based scaffold and then exposing the cell culture to decellularization to deposit acellular matrix (ACM) coatings on the scaffold. This natural biomaterial-based, cell-derived ACM-coated neural scaffold, as a novel nerve graft, was used to bridge a 60 mm long nerve gap in a dog sciatic nerve. At 12 months after grafting, behavioral, functional, and histological evaluation indicated that our developed neural scaffold achieved satisfactory regenerative outcomes, which were very close to those achieved by autologous nerve grafts, the accepted golden standard for peripheral nerve repair. Moreover, additional therapeutic benefits produced by the modification of a neural scaffold with BMSC-derived ACM may be associated with the unique neural activity of the ACM, as evidenced by in vitro experimental findings that the ACM significantly enhanced axonal regrowth and Schwann cell proliferation. Our results will provide a further experimental basis for the translation of ACM-containing neural scaffolds into the clinic.