Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform
Identification of Mycobacterium abscessus species and subspecies using the Cas12a/sgRNA-based nucleic acid detection platform
复制标题
基于Cas12a/sgRNA的核酸检测平台鉴定脓肿分枝杆菌种和亚种
DOI：
10.1007/s10096-019-03757-y
复制
发表时间：
2019-11-28
影响因子：
4.5
通讯作者：
Zhang, Guoliang
中科院分区：
文献类型：
--
作者：
Xiao, Guohui;Zhang, Su;Zhang, Guoliang
快速生长的脓肿分枝杆菌是临床上重要的引起肺部和皮肤疾病的微生物。脓肿支原体复合体由三个亚种组成：脓肿支原体亚种；脓肿，脓肿分枝。马尾毛，和脓肿支原体。bolletii。在此，我们旨在建立一个基于Cas12a/ sgrna的核酸检测平台来鉴定脓肿支原体的种和亚种。通过设计针对rpoB和erm的特异性sgRNA探针（41），我们证明了M.脓肿可以与其他主要分枝杆菌种类区分开来，并在亚种水平上进行鉴定。利用该平台共分离出38株临床脓肿支原体，其中18株为脓肿支原体亚种。20个为脓肿分枝杆菌。massiliense。我们认为，基于Cas12a/ sgrna的核酸检测平台为脓分枝杆菌种和亚种的鉴定提供了一种简单、快速、经济的方法。
The rapidly growing mycobacterium Mycobacterium abscessus is a clinically important organism causing pulmonary and skin diseases. The M. abscessus complex is comprised of three subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. Here, we aimed to develop a Cas12a/sgRNA-based nucleic acid detection platform to identify M. abscessus species and subspecies. By designing specific sgRNA probes targeting rpoB and erm(41), we demonstrated that M. abscessus could be differentiated from other major mycobacterial species and identified at the subspecies level. Using this platform, a total of 38 clinical M. abscessus isolates were identified, 18 as M. abscessus subsp. abscessus and 20 as M. abscessus subsp. massiliense. We concluded that the Cas12a/sgRNA-based nucleic acid detection platform provides an easy-to-use, quick, and cost-effective approach for identification of M. abscessus species and subspecies.