MICRONEUTRALIZATION TESTS FOR SEROLOGICAL TYPING AND SUBTYPING OF FOOT-AND-MOUTH-DISEASE VIRUS-STRAINS
MICRONEUTRALIZATION TESTS FOR SEROLOGICAL TYPING AND SUBTYPING OF FOOT-AND-MOUTH-DISEASE VIRUS-STRAINS
复制标题
DOI：
10.1017/s002217240005381x
复制
发表时间：
1978-01-01
影响因子：
--
通讯作者：
PAY, TWF
中科院分区：
文献类型：
--
作者：
RWEYEMAMU, MM;BOOTH, JC;PAY, TWF
描述了一种用于口蹄疫病毒血清分型的微量中和试验。这是基于早期的观察，剂量反应关系的量子微量中和往往偏离线性。因此，所述分型试验使用未稀释的病毒制剂。在约90%的样本中获得阳性分型，而补体结合试验的阳性分型率约为50%。该检测试剂盒还对极少量的异型病毒污染敏感。对于菌株分化，微量中和试验作为棋盘试验进行。与补体结合试验相比，该试验具有更高的特异性。强调了利用病毒中和试验系统比较口蹄疫病毒株的必要性，特别是为了疫苗选择的目的。应用二维微量中和试验对欧洲、南美、中东和东非的口蹄疫病毒疫苗株进行了比较研究。
A microneutralization test for serotyping of FMD [foot-and-mouth disease] viruses is described. It is based on an earlier observation that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also susceptible to minimal quantities of heterotypic viral contamination. For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was more specific. The necessity to utilize virus-neutralization test systems for comparing FMD virus strains particularly for the purpose of vaccine selection is emphasized. The 2 dimensional microneutralization test was applied to a study of comparing FMD virus vaccine strains for Europe, South America, the Middle East and East Africa.