Noble tandem-repeat galectin of Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni
Noble tandem-repeat galectin of Manila clam Ruditapes philippinarum is induced upon infection with the protozoan parasite Perkinsus olseni
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DOI：
10.1016/j.dci.2008.03.002
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Cho, Moonjae
中科院分区：
文献类型：
--
作者：
Kim, Jin Young;Kim, Young Mee;Cho, Moonjae
凝集素的半乳糖凝集素家族在脊椎动物和无脊椎动物的先天免疫系统中起着至关重要的作用。从海洋无脊椎动物菲律宾蛤仔（Ruditapes philippinarum）中克隆了一种稀有半乳糖凝集素（MCGal）。该蛋白的开放阅读框为918个核苷酸，含309个氨基酸残基，预测分子量为33.9kDa。与其他半乳糖凝集素类似，MCGal既没有信号肽也没有跨膜结构域，但它含有串联重复的碳水化合物识别结构域（CRD），具有对碳水化合物识别重要的典型保守基序。如通过半抗原抑制血凝所确定的，重组MCGal（rMCGal）的碳水化合物识别揭示了rMCGal具有半乳糖凝集素家族共有的特征，即，对半乳糖和N-乙酰半乳糖胺具有显著亲和力。MCGal mRNA主要在心脏、外套膜、足、内收肌、须和虹吸管组织中表达。使用抗MCGal抗体的免疫组织化学（IHC）证实了MCGal在这些组织和血细胞中的表达。与未受到挑战的健康蛤相比，在Perkinsus或弧菌属物种的挑战马尼拉蛤中MCGal mRNA的时间表达上调。rMCGal凝集Vibrio tapetis，并且通过与α-乳糖孵育来抑制凝集。rMCGal也结合到奥氏帕金虫的表面。MCGal在马尼拉蛤的防御中起着至关重要的作用，特别是在病原体识别方面。(C)2008爱思唯尔有限公司保留所有权利。
The galectin family of lectins plays crucial roles in the innate immunity systems of vertebrates and invertebrates. Noble galectin (MCGal) was cloned from the marine invertebrate Ruditapes philippinarum and characterized. This protein has an open reading frame of 918 nucleotides, with 309 amino acid residues, and a predicted molecular weight of 33.9kDa. Similar to other galectins, MCGal has neither a signal peptide nor a transmembrane domain, but it contains tandemly repeated carbohydrate recognition domains (CRDs), with typical conserved motifs that are important for carbohydrate recognition. Carbohydrate recognition by the recombinant MCGal (rMCGal), as determined by hapten inhibition of hemagglutination, revealed that rMCGal has features common to the galectin family, i.e., significant affinity for galactose and N-acetylgalactosamine. MCGal mRNA expression was detected mainly in the heart, mantle, foot, adductor, palp, and siphon tissues. Immunohistochemistry (IHC) using an anti-MCGal antibody confirmed MCGal expression in these tissues and in hemocytes. Temporal expression of MCGal mRNA in Manila clams challenged with Perkinsus or Vibrio species was up-regulated as compared with non-challenged healthy clams. rMCGal agglutinated Vibrio tapetis, and agglutination was inhibited by incubation with alpha-lactose. rMCGal also bound to the surface of Perkinsus olseni. MCGal plays a crucial role in Manila clam defense, particularly with respect to pathogen recognition. (C) 2008 Elsevier Ltd. All rights reserved.