Effects of Trehalose on Flower Senescence from the View Point of Physical States of Water
Effects of Trehalose on Flower Senescence from the View Point of Physical States of Water
复制标题
从水的物理状态来看海藻糖对花卉衰老的影响
DOI：
10.2525/ecb1963.41.3
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
H. Nonami
中科院分区：
文献类型：
--
作者：
M. Iwaya‐Inoue;H. Nonami
用核磁共振氢谱和等压干湿球湿度计研究了海藻糖对球根切花水分物理状态的影响。100和50 mM海藻糖处理显着延长瓶插寿命唐菖蒲和郁金香切花，分别。与对照组织相比，海藻糖保持了较高程度的含水量，同时抑制了蛋白质降解和膜完整性。两种组织中细胞内水的自旋-晶格弛豫时间（T1）的长部分表明海藻糖具有保护液泡水的功能。此外，海藻糖的影响，以提高水的吸收到花被片组织，但不促进细胞伸长，不引起渗透调节，保持高水平的膨压。另一方面，自旋自旋弛豫时间（T2）估计为结合水的短馏分表明，细胞质水也保持。这些结果表明，海藻糖既不作为能量来源，也不直接参与渗透调节，这些特征将其与其他糖（如蔗糖）区分开来。T1、T2和膨压测定表明，在唐菖蒲和郁金香切花中，海藻糖对防止失水有重要作用。
A role of trehalose on physical states of water in cut bulbous flowers was determined by1H-NMR spectroscopy and the isopiestic psychrometer. Treatments with 100 and 50 mM trehalose markedly prolonged vase-life in gladiolus and tulip cut flowers, respectively. Trehalose maintained a higher degree of the water content accompanying with suppressing protein degradation and membrane integrity than the control tissues. The long fraction of spin-lattice relaxation time (T1) of the intracellular water in both tissues indicated that trehalose functioned to protect vacuolar water. Furthermore, trehalose affected to enhance water uptake into tepal tissues though not to promote cell elongation and not to cause osmotic adjustment with maintaining a high level of turgor. On the other hand, the short fraction of spinspin relaxation time (T2) estimated as bound water suggested that cytoplasmic water was also maintained. From these results, it suggests that trehalose does not act as an energy source nor directly participate in osmotic adjustment and these characteristics distinguish it from other sugars such as sucrose. In cut gladiolus and tulip flowers trehalose played an important role to prevent loss of water determined byT1, T2and turgor measurements.
影响因子：
3
作者：
Eason, JR;Ryan, DJ;O'Donoghue, EM
通讯作者：
O'Donoghue, EM