Improvement of Bioethanol Productivity of Immobilized Saccharomyces Bayanus with Using Sodium Alginate-Graft-Poly(N-Vinyl-2-Pyrrolidone) Matrix
Improvement of Bioethanol Productivity of Immobilized Saccharomyces Bayanus with Using Sodium Alginate-Graft-Poly(N-Vinyl-2-Pyrrolidone) Matrix
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DOI：
10.1007/s12010-012-9770-0
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发表时间：
2012-06
影响因子：
3
通讯作者：
Murat Inal;Mustafa Yiǧitoǧlu
中科院分区：
文献类型：
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作者：
Murat Inal;Mustafa Yiǧitoǧlu
本研究对海藻酸钠-接枝-聚(N-乙烯基-2-吡咯烷酮；NaAlg-g-PVP)基质中固定化贝氏酵母的固定化条件和生物乙醇生产特性进行了研究。与氯化钙交联的基质用于固定S。巴亚努斯。与传统的海藻酸钠基质相比，NaAlg-g-PVP 基质的生物乙醇生产率从 4.21 gL−1h−1 增加到 4.84 gL−1h−1。生物乙醇的生产受到初始葡萄糖浓度和发酵培养基中固定化细胞珠百分比的影响。生物乙醇生产率从 3.62 gL−1h−1 增加到 4.84 gL−1h−1，同时葡萄糖浓度从 50 gL−1 增加到 100 gL−1。由于发酵培养基中固定化细胞珠的百分比从 10% 增加到 20%，生物乙醇生产率从 4.84 gL−1h−1 增加到 8.68 gL−1h−1。经过六次重复发酵后，细胞固定的 NaAlg-g-PVP 珠子的初始活性得到了 92% 的保护。
In this study, immobilization conditions and bioethanol production characteristics of immobilizedSaccharomyces bayanuswere investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization ofS. bayanus. Bioethanol productivity of the NaAlg-g-PVP matrix was found to increase from 4.21 to 4.84 gL−1h−1when compared with the convential sodium alginate matrix. The production of bioethanol was affected by initial glucose concentration and percentage of immobilized cell beads in fermentation medium. Bioethanol productivity was increased from 3.62 to 4.84 gL−1h−1while the glucose concentration increasing from 50 to 100 gL−1. Due to the increase in percentage from 10 to 20 % of immobilized cell beads in the fermentation medium, bioethanol productivity was increased from 4.84 to 8.68 gL−1h−1. The cell immobilized NaAlg-g-PVP beads were protected 92 % of initial activity after six repeated fermentation.