Calligraphy of Nanoplasmonic Bioink-Based Multiplex Immunosensor for Precision Immune Monitoring and Modulation
Calligraphy of Nanoplasmonic Bioink-Based Multiplex Immunosensor for Precision Immune Monitoring and Modulation
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DOI：
10.1021/acsami.3c11417
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发表时间：
2023-10-19
影响因子：
9.5
通讯作者：
Chen，Pengyu
中科院分区：
文献类型：
--
作者：
He，Jiacheng;Wu，Siqi;Chen，Pengyu
免疫调节疗法最近在治疗免疫相关疾病如癌症和病毒感染方面引起了极大的兴趣。对免疫调节剂的新一轮热情主要围绕细胞因子，刺激了对新型免疫监测和诊断工具的新兴需求和机会。考虑到高度动态的免疫状态和有限的治疗干预窗口，细胞因子的精确实时检测对于有效监测和管理免疫系统并优化治疗结果至关重要。这种快速、灵敏、多重免疫分析平台的临床成功进一步要求该系统易于集成和制造，以用于样品保留和大规模生产，从而进行大规模并行分析。在这篇文章中，我们开发了一种基于纳米等离子体的生物墨水，无标记，多重免疫传感器，可以很容易地“写”到玻璃基板上通过一步书法图案。这种简单的纳米光刻技术允许在1分钟内对最少3 μL的纳米等离子体生物墨水进行可编程的图案化，从而能够通过2小时的简单孵育来制造纳米等离子体微阵列免疫传感器。所开发的免疫传感器成功地应用于多种细胞因子（例如，白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β（TGF-β））。该集成平台协同整合了纳米合成、纳米纤维和纳米生物传感的概念，在无标记多重免疫传感装置的可规模化生产中显示出巨大的潜力，该装置具有上级分析性能，可用于免疫诊断和免疫治疗的临床应用。
Immunomodulation therapies have attracted immense interest recently for the treatment of immune-related diseases, such as cancer and viral infections. This new wave of enthusiasm for immunomodulators, predominantly revolving around cytokines, has spurred emerging needs and opportunities for novel immune monitoring and diagnostic tools. Considering the highly dynamic immune status and limited window for therapeutic intervention, precise real-time detection of cytokines is critical to effectively monitor and manage the immune system and optimize the therapeutic outcome. The clinical success of such a rapid, sensitive, multiplex immunoanalytical platform further requires the system to have ease of integration and fabrication for sample sparing and large-scale production toward massive parallel analysis. In this article, we developed a nanoplasmonic bioink-based, label-free, multiplex immunosensor that can be readily “written” onto a glass substrate via one-step calligraphy patterning. This facile nanolithography technique allows programmable patterning of a minimum of 3 μL of nanoplasmonic bioink in 1 min and thus enables fabrication of a nanoplasmonic microarray immunosensor with 2 h simple incubation. The developed immunosensor was successfully applied for real-time, parallel detection of multiple cytokines (e.g., interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta (TGF-β)) in immunomodulated macrophage samples. This integrated platform synergistically incorporates the concepts of nanosynthesis, nanofabrication, and nanobiosensing, showing great potential in the scalable production of label-free multiplex immunosensing devices with superior analytical performance for clinical applications in immunodiagnostics and immunotherapy.