Soft freezing-induced self-assembly of silk fibroin for tunable gelation
Soft freezing-induced self-assembly of silk fibroin for tunable gelation
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软冷冻诱导丝素蛋白自组装可调节凝胶化
DOI：
10.1016/j.ijbiomac.2018.05.223
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发表时间：
2018-10-01
影响因子：
8.2
通讯作者：
Wang, Dong
中科院分区：
文献类型：
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作者：
Li, Xiufang;Yan, Shuqin;Wang, Dong
丝素（SF）水凝胶是生物材料领域的一个很有前途的候选材料；但胶凝时间长，限制了其应用。在本研究中，我们开发了一种名为软冷冻的新策略来加速SF蛋白的过程并控制其溶胶-凝胶转变。通过软冷冻诱导SF蛋白自组装，获得具有亚稳态结构的重建SF溶液。发现软冻结过程触发了结构从随机结构向有序富结构构象的转变。凝胶动力学表明，SF蛋白的凝胶时间可以通过改变冷冻时间和初始浓度来调节。即使在极低的浓度下，重建的SF溶液也能在6小时内增强溶胶-凝胶转变。这里描述的方法的吸引人的特点包括加速凝胶，不含化学剂，降低加工复杂性。凝胶时间短的SF溶液可用于组织工程和再生医学的细胞包封和注射应用，大大扩展了SF水凝胶的应用范围。(C) 2018 Elsevier B.V.版权所有
Silk fibroin (SF) hydrogel is a promising candidate in biomaterial field; however its application is quite limited by long-gelation time. In the present study, we developed a novel strategy named soft freezing to accelerate the process and control the sol-gel transition of SF protein. SF protein was induced to self-assembly by soft freezing process for achieving the reconstructed SF solution with metastable structure. It was found that the soft freezing process triggers the structural transition from random structure to ordered structure-rich conformation. Gelation kinetics showed that the gelation time of SF protein could be regulated by changing freezing time and initial concentration. The reconstructed SF solution allowed enhanced sol-gel transition within 6 hours, even at extremely low concentration. The attractive features of the method described here include the accelerated gelation, free of chemical agents, and reducing processing complexity. The SF solution with short gelation time will be applicable as cell encapsulation and injectable applications for tissue engineering and regenerative medicine, which greatly expand the applications of SF hydrogels. (C) 2018 Elsevier B.V. All rights reserved.