A new locus for resistance to γ-radiation-induced thymic lymphoma identified using inter-specific consomic and inter-specific recombinant congenic strains of mice
A new locus for resistance to γ-radiation-induced thymic lymphoma identified using inter-specific consomic and inter-specific recombinant congenic strains of mice
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DOI：
10.1038/sj.onc.1205846
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发表时间：
2002-09-26
期刊：
影响因子：
8
通讯作者：
Fernández-Piqueras, J
中科院分区：
文献类型：
--
作者：
Santos, J;Montagutelli, X;Fernández-Piqueras, J
C57BL/6J 近交系小鼠在急性伽玛射线照射后以非常高的频率发展胸腺淋巴瘤，而源自 Mus spretus 物种野生祖先的几种近交系小鼠及其与 C57BL/6J 的 F1 杂交种的小鼠表现出极高的抵抗力。借助种间小鼠品系 (ICS)（在 C57BL/6J 遗传背景上携带单条渐渗的小家鼠染色体）对 γ 辐射诱导的胸腺淋巴瘤 (RITL) 抗性的遗传决定论进行分析，为 19 号染色体上存在胸腺淋巴瘤抗性 (Tlyr1) 基因座提供了重要证据。随后对康体小鼠之间杂交产生的回交后代进行了分析Mus spretus 19 号染色体和 C57BL/6J 小鼠的杂合子，以及对种间重组同类株 (IRCS) 的研究表明，该 Tlyr1 位点位于 D19Mit60-D19Mit40 染色体区间内。除了发现控制 RITL 发展的新基因座之外，我们的研究还强调了 ICS 和 IRCS 对于癌症易感性遗传分析的价值。
Mice of the C57BL/6J inbred strain develop thymic lymphomas at very high frequency after acute gamma-irradiation, while mice of several inbred strains derived from the wild progenitor of the Mus spretus species and their F1 hybrids with C57BL/6J appear extremely resistant. Analysis of the genetic determinism of the gamma-radiation-induced thymic lymphoma (RITL) resistance with the help of inter-specific consomic strains (ICS), which carry a single introgressed Mus spretus chromosome on a C57BL/6J genetic background, provide significant evidence for the existence of a thymic lymphoma resistance (Tlyr1) locus on chromosome 19. The subsequent analysis of the backcross progeny resulting from a cross between consomic mice heterozygous for the Mus spretus chromosome 19 and C57BL/6J mice, together with the study of inter-specific recombinant congenic strains (IRCS), suggest that this Tlyr1 locus maps within the D19Mit60-D19Mit40 chromosome interval. In addition to the discovery of a new locus controlling RITL development, our study emphasizes the value of ICS and IRCS for the genetic analysis of cancer predisposition.