A RECOMBINANT SALMONELLA-TYPHIMURIUM VACCINE INDUCES LOCAL IMMUNITY BY 4 DIFFERENT ROUTES OF IMMUNIZATION
A RECOMBINANT SALMONELLA-TYPHIMURIUM VACCINE INDUCES LOCAL IMMUNITY BY 4 DIFFERENT ROUTES OF IMMUNIZATION
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DOI：
10.1128/iai.63.9.3279-3286.1995
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发表时间：
1995-09-01
影响因子：
3.1
通讯作者：
NARDELLIHAEFLIGER, D
中科院分区：
文献类型：
--
作者：
HOPKINS, S;KRAEHENBUHL, JP;NARDELLIHAEFLIGER, D
用携带编码B型肝炎病毒核心抗原（HBc）的杂合形式的质粒的减毒鼠伤寒沙门氏菌菌株（Phop（c））免疫小鼠诱导针对细菌脂多糖（LPS）和HBc的特异性抗体应答。不同的粘膜免疫途径，即，比较了口服、鼻、直肠和阴道的疫苗在接近或远离免疫部位的部位诱导全身以及粘膜应答的能力。在唾液、粪便、生殖道、支气管和肠道分泌物中检测抗LPS和抗HBc免疫球蛋白A（伊加）抗体。通过所有途径免疫后，观察到血清和分泌物中的特异性抗体;然而，对LPS的反应与对HBc的反应无关。在血清、唾液、生殖道和支气管分泌物中，通过鼻途径免疫获得了高量的抗-HBc伊加，阴道免疫在小鼠中导致两种不同的应答：高和低。我们观察到的特异性免疫应答水平和这些小鼠在免疫时的发情状态之间的相关性，直肠免疫诱导大量的伊加对HBc和LPS在结肠直肠分泌物和粪便，但不是在遥远的网站。这些数据表明S.鼠伤寒杆菌能够侵入不同的粘膜组织，并在单次免疫后诱导针对其自身和所携带抗原的持久局部伊加应答。
Immunization of mice with an attenuated Salmonella typhimurium strain (Phop(c)) carrying a plasmid encoding a hybrid form of the hepatitis B virus core antigen (HBc) induced specific antibody responses against the bacterial lipopolysaccharide (LPS) and HBc. Different mucosal routes of immunization, i.e., oral, nasal, rectal, and vaginal, were compared for their ability to induce a systemic as well as a mucosal response at sites proximal or distant to the site of immunization. Anti-LPS and anti-HBc immunoglobulin A (IgA) antibodies were measured in saliva, in feces, and in genital, bronchial, and intestinal secretions, Specific antibodies in serum and secretions were observed after immunization via all routes; however, the response to LPS was independent of that against HBc. In serum, Saliva, and genital and bronchial secretions, high amounts of anti-HBc IgA were obtained by the nasal route of immunization, Vaginal immunization resulted in two different responses in mice: high and low. We observed a correlation between the level of specific immune response and the estrous status of these mice at the time of immunization, Rectal immunization induced high amounts of IgA against HBc and LPS in colonorectal secretions and feces but not at distant sites. These data suggest that S. typhimurium is able to invade different mucosal tissues and induce long-lasting local IgA responses against itself and a carried antigen after a single immunization.