Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
Sea urchin (Strongylocentrotus intermedius) polysaccharide enhanced BMP-2 induced osteogenic differentiation and its structural analysis
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DOI：
10.1016/j.jff.2015.02.025
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发表时间：
2015-04
影响因子：
5.6
通讯作者：
Jingfeng Yang;Meng Yi;Jinfeng Pan;Zhao Jun;Li-ming Sun;Xinping Lin;Yu Cao;Lu Huang;B. Zhu;Chenxu Yu
中科院分区：
文献类型：
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作者：
Jingfeng Yang;Meng Yi;Jinfeng Pan;Zhao Jun;Li-ming Sun;Xinping Lin;Yu Cao;Lu Huang;B. Zhu;Chenxu Yu
从海胆（Strongylocentrotus intermedius）性腺中提取出一种新型海胆多糖（SUP），并表征其协同增强骨形态发生蛋白-2（BMP-2）诱导成骨细胞分化功能的生物活性。结果表明，该酶为均一酶，平均分子量为4.4kDa，主要由甘露糖和少量葡萄糖及N-乙酰氨基葡萄糖组成，主链为1，4-D-Man和1，3-D-Man交替残基。研究表明，当BMP-2浓度高于10 µg/mL时，BMP-2可协同增强C2 C12成肌细胞中BMP-2诱导的成骨细胞分化，碱性磷酸酶（ALP）活性显著增加。C2 C12成肌细胞的组织化学染色也证实了ALP活性的升高。这些结果表明，芦荟可以促进骨再生，并可能作为一种功能性食品补充剂，为骨骼健康。
A new sea urchin polysaccharide (SUP) was extracted from the gonad of sea urchin (Strongylocentrotus intermedius), and its bioactivity to synergistically enhance the functionality of bone morphogenetic protein-2 (BMP-2) induced osteoblastic differentiation was characterized. Results showed that the SUP is homogenous with an average molecular weight of 4.4 kDa, and it is mainly composed of mannose with a small number of glucose and N-acetylglucosamine, with a backbone of alternating 1, 4-D-Man and 1, 3-D-Man residues. The SUP was shown to synergistically enhance BMP-2 induced osteoblastic differentiation in C2C12 myoblast cells, evidenced by a significant increase in the activity of alkaline phosphatase (ALP), when the concentration of the SUP was higher than 10 µg/mL. The elevated ALP activity was also confirmed by histochemical staining of C2C12 myoblast cells. These results suggest that the SUP can promote bone regeneration and may serve as a functional food supplement for bone health.