A comparison of allogeneic and autologous mesenchymal stromal cells and osteoprogenitor cells in augmenting bone formation around massive bone tumor prostheses
A comparison of allogeneic and autologous mesenchymal stromal cells and osteoprogenitor cells in augmenting bone formation around massive bone tumor prostheses
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DOI：
10.1002/jbm.a.34536
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发表时间：
2013-08-01
影响因子：
4.9
通讯作者：
Blunn, Gordon W.
中科院分区：
文献类型：
--
作者：
Coathup, Melanie J.;Kalia, Priya;Blunn, Gordon W.
在羊模型中，将自体间充质间质细胞（MSCs）喷涂到羟基磷灰石（HA）涂层的生长颈圈上，该颈圈位于大块骨肿瘤植入物的肩部，显著增加了皮质外骨桥接和骨整合。在这项研究中，我们研究了异体间充质干细胞和骨祖细胞（OPCs）与自体间充质干细胞相比对骨生长的促进作用相同的假设。所有项圈都涂了HA。i组仅涂纤维蛋白胶。细胞与纤维蛋白胶结合，植入物获得（ii） 2 × 106个自体MSCs， （iii） 10 × 106个自体MSCs， （iv） 2 × 106个OPCs， (v) 10 × 106个OPCs，或（vi） 10 × 106个异体MSCs。在第七组，项圈仅涂有透明质酸。利用影像学和组织学技术对新生骨面积和骨-种植体与长入环的接触进行量化。结果表明，与其他各实验组相比，喷施同种异体间充质干细胞的各组牙套周围均无骨形成，且新生骨明显增多（p < 0.05）。10 × 106 OPC组骨生长和骨与种植体接触最多。将MSCs或OPCs喷洒到种植体表面可用于患者；然而，需要进一步的工作来确定异体细胞在体内骨增强中的作用。(c) 2012 Wiley期刊有限公司[J]生物医学工程学报，2013。
Spraying autologous mesenchymal stromal cells (MSCs) onto hydroxyapatite (HA)-coated ingrowth collars, located at the shoulder of massive bone tumor implants, significantly increased extracortical bone-bridging and osteointegration in an ovine model. In this study, we investigated the hypothesis that allogeneic MSCs and osteoprogenitor cells (OPCs) will augment bone growth equally when compared with autologous BMSCs. All collars were HA coated. In group i, the HA collar was coated with fibrin glue only. Cells were combined with fibrin glue and implants received (ii) 2 x 106 autologous MSCs, (iii) 10 x 106 autologous MSCs, (iv) 2 x 106 OPCs, (v) 10 x 106 OPCs, or (vi) 10 x 106 allogeneic MSCs. In group vii, collars were HA coated only. New bone area and bone-implant contact onto the ingrowth collar was quantified radiographically and using histological techniques. Results showed that no extracortical bone formed adjacent to any collars sprayed with allogeneic MSCs and significantly more new bone was measured when all other experimental groups were compared (p < 0.05 in all cases). Most bone growth and bone-implant contact occurred in the 10 x 106 OPC group. Spraying MSCs or OPCs onto the implant surface may be used in patients; however, further work is needed to determine the role of allogeneic cells in bone augmentation in vivo. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.