Fluorescence spectroscopic imaging to detect changes in collagen and elastin following laser tissue welding
Fluorescence spectroscopic imaging to detect changes in collagen and elastin following laser tissue welding
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DOI：
10.1089/clm.2000.18.3
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发表时间：
2000-02-01
期刊：
影响因子：
--
通讯作者：
Alfano, RR
中科院分区：
文献类型：
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作者：
Tang, J;Zeng, FN;Alfano, RR
目的：评价天然荧光成像技术在原位分子检测中的应用。背景资料摘要：没有理想的非侵入性方法可以在不切除组织进行组织学检查的情况下监测激光焊接接头局部区域的分子变化。方法：用钛宝石激光(800 Nm)将两片动物皮肤焊接在一起，分别在340mN和380mN激发波长下对特定发射波长(C)处的胶原和450 nm处的弹性蛋白进行荧光成像。结果：在荧光图像上，焊接区的胶原和弹性蛋白释放减少。这些结果与偏振光下的天狼星红F3BA和地衣红染色的组织学结果相一致。结论：光学光谱成像为激光组织焊接的微观评价提供了一种新的无创检测方法。
Objective: A study was performed to evaluate the use of native fluorescence imaging to detect in situ molecular changes. Summary background data: There is no ideal noninvasive method to monitor molecular changes in a local region at a laser weld joint without removing a section of tissue for histological examination. Methods: Two sections of animal skin were welded together border to border using a Ti:sapphire laser beam (800 nm), Fluorescence imaging was performed on the cross section of the welded site at specific emission wavelengths (lambda(c)) for collagen at 380 nm and for elastin at 450 nm using excitation wavelengths (lambda(e)) of 340 mn, and 380 mn, respectively. Results: A reduction of the collagen and elastin emission was observed in the fluorescence images of the welded region. These results were confirmed with histology using picrosirius red F3BA under polarized light and orcein stains. Conclusion: Optical spectroscopic imaging offers a new noninvasive detecting method for microscopic evaluation of laser tissue welding.