Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.
Decrease of hindpaw withdrawal latency by cocaine- and amphetamine-regulated transcript peptide to the mouse spinal cord.
复制标题
可卡因和安非他明调节的转录肽对小鼠脊髓的后爪缩回潜伏期减少。
DOI：
10.1016/s0014-2999(00)00374-5
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发表时间：
2000
影响因子：
5
通讯作者：
Dun,NJ
中科院分区：
文献类型：
--
作者：
Ohsawa,M;Dun,SL;Tseng,LF;Chang,J;Dun,NJ
使用针对可卡因和安非他明调节转录物 (CART) 肽 (55-102) 的抗血清进行的免疫组织化学研究显示，小鼠背角层 I 和 II 中存在丰富的 CART 免疫反应性纤维。一些CART阳性体细胞散布在背角和中央管周围。鞘内注射 3、10 和 100 ng 剂量的 CART 肽-(55-102) 导致缩爪潜伏期出现剂量依赖性且显着降低；而注射生理盐水则无明显效果。我们的结果提供了第一个证据，证明 CART 免疫反应性纤维存在于背角中，并且通过小鼠缩爪潜伏期评估，鞘内施用的肽会产生痛觉过敏。
Immunohistochemical studies with the use of an antiserum against the cocaine- and amphetamine-regulated transcript (CART) peptide-(55–102) showed an abundance of CART-immunoreactive fibers in the mouse dorsal horn laminae I and II. A few CART-positive somata were scattered in the dorsal horn and around the central canal. Intrathecal injection of the CART peptide-(55–102) at doses 3, 10 and 100 ng caused a dose-dependent and significant decrease of paw withdrawal latency; whereas, saline injection was without significant effect. Our results provide the first evidence that CART-immunoreactive fibers are present in the dorsal horn and that the peptide administered intrathecally produces hyperalgesia, as assessed by paw withdrawal latency in mice.