Ginsenoside Rg1 ameliorates palmitic acid-induced insulin resistance in HepG2 cells in association with modulating Akt and JNK activity
Ginsenoside Rg1 ameliorates palmitic acid-induced insulin resistance in HepG2 cells in association with modulating Akt and JNK activity
复制标题
人参皂苷 Rg1 改善 HepG2 细胞中棕榈酸诱导的胰岛素抵抗，并与调节 Akt 和 JNK 活性相关
DOI：
10.1016/j.pharep.2019.07.004
复制
发表时间：
2019-12-01
影响因子：
4.4
通讯作者：
Chen, Peng
中科院分区：
文献类型：
--
作者：
Mo, Jiao;Zhou, Yulin;Chen, Peng
背景：过量摄入饱和脂肪可诱导肝脏胰岛素抵抗。人参皂苷Rg 1（Ginsenoside Rg 1，GRg 1）是人参中富含的主要活性成分，对肝脏疾病有一定的缓解作用。方法：采用0.5mM棕榈酸（PA）诱导HepG 2细胞胰岛素抵抗24 h，观察GRg 1对细胞葡萄糖消耗的影响。用Western blot和实时定量聚合酶链反应分析磷酸烯醇式丙酮酸羧激酶（PEPCK）和葡萄糖-6-磷酸（G6-phosphate，G6-phosphate）的表达。通过测量蛋白磷酸化来分析蛋白激酶和转录因子的活化。结果：GRg 1通过下调HepG 2细胞葡萄糖代谢相关基因G6 pase和PEPCK，逆转PA诱导的HepG 2细胞葡萄糖消耗减少。GRg 1在PA攻击的HepG 2细胞中增加Akt活化，但抑制JNK活化。结论：GRg 1通过维持胰岛素信号敏感性，保护肝脏胰岛素抵抗，是一种很有前途的替代药物。(C)2019年爱思唯尔出版
Background: Hepatic insulin resistance can be induced by excess dietary intake of saturated fat. Ginsenoside Rg1 (GRg1), the major active ginsenoside enriched in tonic food ginseng, was reported to help alleviate liver diseases. In the present study, GRg1 was evaluated for its impact on palmitic acid (PA)-induced hepatic insulin resistance model in vitro.Methods: Insulin resistance in HepG2 cells was induced by 0.5 mM PA exposure for 24 h and then the effect of GRg1 on cellular glucose consumption was measured. Expression of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphate (G6Pase) were analyzed by Western blot and quantitative real-time polymerase chain reaction. Activation of protein kinases and transcript factor was analyzed by measuring protein phosphorylation. The influence of GRg1 on reactive oxygen species (ROS) production in HepG2 was also examined.Results: GRg1 reversed PA-induced decrease in glucose consumption of HepG2 cells by downregulating gluconeogenesis genes G6pase and PEPCK. GRg1 increased Akt activation but inhibited JNK activation in PA-challenged HepG2 cells. Cellular ROS level was elevated in insulin-resistant HepG2 cells but was reduced by GRg1.Conclusions: Together these findings indicate that GRg1 protects against hepatic insulin resistance via preserving insulin signaling sensitivity and is a promising alternative medicine. (C) 2019 Published by Elsevier B.V.