Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone
Identification of blaLAP-2 and qnrS1 genes in the internationally successful Klebsiella pneumoniae ST147 clone
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DOI：
10.1099/jmm.0.050542-0
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发表时间：
2013-02-01
影响因子：
3
通讯作者：
Mazzariol, Annarita
中科院分区：
文献类型：
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作者：
Kocsis, Bela;Kocsis, Erika;Mazzariol, Annarita
我们调查了26株肠杆菌科细菌中存在的β-内酰胺酶基因（bla），已发现qnrS 1基因阳性，qnrS 1基因是质粒介导的喹诺酮类耐药决定簇。三株K. 2008-2009年期间在维罗纳大学医院分离的肺炎链球菌对窄谱β-内酰胺酶β-内酰胺酶2呈阳性。这些菌株，即VRB 586、VRE 185和VRE 196，分别从来自不同单位的不同患者的尿液、胆汁和腹膜引流物中培养。利用ISEcl 2技术将bla（β-2）和qnrS 1抗性决定基因分离并定位在一个97 kb的可接合的、不可分型的质粒上，该质粒可转移到受体菌株E. coli J53。氟喹诺酮和头孢他啶的MIC增加1-2倍，在transconjugant细胞。通过PFGE发现三种肺炎克雷伯菌菌株是克隆的，并通过MLST鉴定为属于ST 147，这是一种国际上成功的克隆。克隆了克雷伯氏菌ISEcl 2和qnrS 1基因的质粒序列。pneumoniae ST 147与先前检测到的携带qnrS 1的质粒高度相似，这表明该质粒具有稳定的遗传结构，并且这些耐药决定簇具有共同的来源。据我们所知，这是国际上首次成功报道的携带bla（β-2）和qnrS 1基因的肺炎克雷伯菌ST 147菌株，也是意大利首例β-内酰胺酶。
We investigated the presence of beta-lactamase genes (bla) in 26 strains of Enterobacteriaceae already found positive for the qnrS1 gene, a plasmid-mediated quinolone resistance determinant. Three strains of K. pneumoniae, isolated in the period 2008-2009 at the University Hospital in Verona, were positive for LAP-2, a narrow-spectrum beta-lactamase. These strains, namely VRB586, VRE185 and VRE196, were cultured from urine, bile and peritoneal drainage, respectively, of different patients from different units. The bla(LAP-2) and qnrS1 resistance determinant genes were separated by ISEcl2 and were located on a 97 kb conjugable and untypable plasmid, which could be transferred to a recipient strain, E. coli J53. The fluoroquinolone and ceftazidime MICs increased 1-2-fold in the transconjugant cells. The three K pneumoniae strains were found to be clonal by PFGE and were identified as belonging to ST147, an internationally successful clone, by MLST. The plasmid sequence, including ISEcl2 and qnrS1 genes, of K. pneumoniae ST147 was found to be highly similar to previously detected qnrS1-harbouring plasmids, suggesting the plasmid has a stable genetic structure and that these resistance determinants have a common source. To the best of our knowledge, this is the first report of the internationally successful K pneumoniae ST147 strain carrying bla(LAP-2) and qnrS1 genes and is the first case of LAP beta-lactamase in Italy.