Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species
Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species
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DOI：
10.1007/s11010-006-9170-2
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发表时间：
2006-10-01
影响因子：
4.3
通讯作者：
Suguna, Lonchin
中科院分区：
文献类型：
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作者：
Panchatcharam, Manikandan;Miriyala, Sumitra;Suguna, Lonchin
伤口愈合包括重建受损组织完整性的有序进展。多种天然产品已被证明可以加速愈合过程。本研究旨在确定姜黄素对大鼠皮肤伤口愈合过程中胶原蛋白特性和抗氧化特性变化的作用。在大鼠背部制作全层切除伤口并局部施用姜黄素。伤后第4、8、12天取出的创面组织用于分析生化和病理变化。姜黄素可增加伤口部位的细胞增殖和胶原蛋白合成，伤口组织 DNA、总蛋白和 III 型胶原蛋白含量的增加就证明了这一点。发现姜黄素处理的伤口愈合得更快，如上皮形成率提高、伤口收缩和拉伸强度增加，这也通过组织病理学检查得到证实。姜黄素治疗可降低脂质过氧化物 (LP) 水平，同时超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GPx) 活性显着增加，显示出姜黄素在加速伤口愈合方面的抗氧化特性。通过增加酸溶性胶原蛋白的稳定性、醛含量、收缩温度和拉伸强度，在姜黄素处理的大鼠中观察到胶原蛋白更好的成熟和交联。结果清楚地证实了局部应用姜黄素对加速伤口愈合及其抗氧化作用的有益作用。
Wound healing consists of an orderly progression of events that re-establish the integrity of the damaged tissue. Several natural products have been shown to accelerate the healing process. The present investigation was undertaken to determine the role of curcumin on changes in collagen characteristics and antioxidant property during cutaneous wound healing in rats. Full-thickness excision wounds were made on the back of rat and curcumin was administered topically. The wound tissues removed on 4th, 8th and 12th day (post-wound) were used to analyse biochemical and pathological changes. Curcumin increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and type III collagen content of wound tissues. Curcumin treated wounds were found to heal much faster as indicated by improved rates of epithelialisation, wound contraction and increased tensile strength which were also confirmed by histopathological examinations. Curcumin treatment was shown to decrease the levels of lipid peroxides (LPs), while the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), activities were significantly increased exhibiting the antioxidant properties of curcumin in accelerating wound healing. Better maturation and cross linking of collagen were observed in the curcumin treated rats, by increased stability of acid-soluble collagen, aldehyde content, shrinkage temperature and tensile strength. The results clearly substantiate the beneficial effects of the topical application of curcumin in the acceleration of wound healing and its antioxidant effect.