Chemosensory proteins of Locusta migratoria
Chemosensory proteins of Locusta migratoria
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DOI：
10.1046/j.1365-2583.2003.00394.x
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发表时间：
2003-04-01
影响因子：
2.6
通讯作者：
Pelosi, P
中科院分区：
文献类型：
--
作者：
Ban, L;Scaloni, A;Pelosi, P
基于飞蝗触角一系列不同 cDNA 的分子克隆，已鉴定出飞蝗中两种不同类别的化学感应蛋白 (CSP)。已经从触角和翅提取物中纯化了几种 CSP 亚型并进行了生化表征，其中一些亚型对应于已鉴定的 cDNA 预测的表达产物。在翅膀中，通过气相色谱-质谱法将与这些蛋白质结合的主要内源配体的性质确定为油酰胺。其中一种亚型已在细菌系统中高产表达并用于荧光结合测定。竞争性结合实验表明长链化合物是最好的配体之一。
Two different classes of chemosensory proteins (CSPs) in Locusta migratoria have been identified on the basis of the molecular cloning of a series of different cDNAs from the antennae of this insect. Several CSP isoforms have been purified and biochemically characterized from antennal and wing extracts, some of them corresponding to expression products predicted for the identified cDNAs. In wings, the nature of the main endogenous ligand binding to these proteins was determined as oleoamide by a gas chromatography-mass spectrometric approach. One of these isoforms has been expressed in a bacterial system with high yield and used in a fluorescent binding assay. Competitive binding experiments have indicated the presence of long-chain compounds among the best ligands.