Cultured granulosa cells produce two plasminogen activators and an antiactivator, each regulated differently by gonadotropins.
Cultured granulosa cells produce two plasminogen activators and an antiactivator, each regulated differently by gonadotropins.
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DOI：
10.1210/endo-116-4-1666
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发表时间：
1985-04
期刊：
影响因子：
4.8
通讯作者：
T. Ny;L. Bjersing;A. Hsueh;D. Loskutoff
中科院分区：
文献类型：
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作者：
T. Ny;L. Bjersing;A. Hsueh;D. Loskutoff
尽管用促性腺激素处理培养的颗粒细胞会增加其纤溶活性，但这种作用的生化性质尚不清楚。我们使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和纤维蛋白自显影技术来表征颗粒细胞分泌的纤溶成分。这些细胞的纤溶活性源自组织型纤溶酶原激活剂（t-PA）和尿激酶样激活剂（u-PA）的产生。这些细胞还产生纤维蛋白溶解抑制剂（抗激活剂）。 FSH 和 LH 刺激 t-PA 活性并抑制抗激活剂活性，而 u-PA 活性不受促性腺激素影响。促性腺激素对这些分子的差异调节可能对于排卵至关重要。
Although treatment of cultured granulosa cells with gonadotropins increases their fibrinolytic activity, the biochemical nature of this effect is unclear. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fibrin autography techniques to characterize the fibrinolytic components secreted by granulosa cells. The fibrinolytic activity of these cells results from the production of both a tissue-type plasminogen activator (t-PA) and a urokinase-like activator (u-PA). The cells also produce an inhibitor of fibrinolysis (antiactivator). FSH and LH stimulate t-PA activity and suppress antiactivator activity, while u-PA activity is not affected by the gonadotropins. The differential regulation of these molecules by the gonadotropins may be essential for ovulation.