Dietary Cellulose Supplementation Modulates the Immune Response in a Murine Endotoxemia Model.
Dietary Cellulose Supplementation Modulates the Immune Response in a Murine Endotoxemia Model.
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饮食中补充纤维素调节鼠内毒素血症模型中的免疫反应。
DOI：
10.1097/shk.0000000000001180
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发表时间：
2019-04
期刊：
影响因子：
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通讯作者：
Aneja RK
中科院分区：
文献类型：
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作者：
Di Caro V;Cummings JL;Alcamo AM;Piganelli JD;Clark RSB;Morowitz MJ;Aneja RK
膳食纤维在慢性炎症性疾病中的作用已被探索，但对其在急性炎症中的益处知之甚少。以前，我们已经证明，膳食纤维素补充剂通过促进与代谢健康相关的肠道微生物群的生长，在败血症小鼠模型中提供保护。存活益处与促炎细胞因子的血清浓度降低、肺中中性粒细胞浸润减少和肝脏炎症减轻相关。在这里，我们的目标是了解操纵肠道微生物组的益处是否在致死性小鼠内毒素血症模型中对免疫系统产生更广泛的“全身”影响。我们假设，小鼠喂养高纤维纤维素（HF）饮食将证明减少活化的巨噬细胞和树突状细胞（DC）和T调节细胞（T细胞）对T细胞反应性的抑制能力的同时增加。我们的特点是免疫概况和激活状态的巨噬细胞，树突状细胞和T细胞在HF饮食的小鼠，然后受到内毒素血症。补充HF饮食降低LPS给药后小鼠脾巨噬细胞和DC的数量和活化。类似地，与常规饮食的小鼠相比，HF饮食放大了T细胞的抑制功能并诱导T细胞无反应性。我们的数据表明，在鼠内毒素血症模型中，HF饮食的使用可以是一种简单而有效的工具，其降低了NF-κB的肝脏DNA结合活性，导致促炎细胞因子反应的降低。
The role of dietary fiber in chronic inflammatory disorders has been explored, but very little is known about its benefits in acute inflammation. Previously, we have demonstrated that dietary cellulose supplementation confers protection in a murine model of sepsis by promoting the growth of the gut microbiota that are linked to metabolic health. The survival benefit is associated with a decrease in serum concentration of pro-inflammatory cytokines, reduced neutrophil infiltration in the lungs, and diminished hepatic inflammation. Here we aim to understand if the benefit of manipulating the gut microbiome exerts a broader “systemic” influence on the immune system in a lethal murine endotoxemia model. We hypothesize that mice fed high fiber cellulose (HF) diet will demonstrate a reduction in activated macrophages and dendritic cells (DCs) and a concomitant increase in the suppressive capacity of T regulatory cells (Tregs) toward T cells responsiveness. We characterized the immunological profile and activation status of macrophages, DCs and T cells in mice on HF diet who were then subjected to endotoxemia. Supplementation with HF diet decreased the number and activation of splenic macrophages and DCs in mice after LPS administration. Similarly, HF diet amplified the suppressive function of Tregs and induced anergy in T cells as compared to mice on a regular diet. Our data suggest that the use of HF diet can be a simple, yet effective tool that decreases the hepatic DNA binding activity of NF-κB leading to a reduction in pro-inflammatory cytokine response in a murine endotoxemia model.