Acute choline supplementation in vivo enhances acetylcholine synthesis in vitro when neurotransmitter release is increased by potassium.
Acute choline supplementation in vivo enhances acetylcholine synthesis in vitro when neurotransmitter release is increased by potassium.
复制标题
当钾增加神经递质释放时，体内急性胆碱补充可增强体外乙酰胆碱的合成。
DOI：
10.1111/j.1471-4159.1989.tb09157.x
复制
发表时间：
1989
影响因子：
4.7
通讯作者：
Rothermel,S
中科院分区：
文献类型：
--
作者：
Wecker,L;Cawley,G;Rothermel,S
The main objective of these studies was to determine whether the acute administration of choline to rats provides supplemental precursor that can be used to support acetylcholine synthesis when the demand for choline is increased by increasing neurotransmitter release. For these experiments, hippocampal and striatal slices were prepared from rats that had received saline or an acute injection of choline. Slices were incubated in a choline‐free buffer containing 4.74–35 mMKC, and acetylcholine synthesis and release and choline production were measured. The initial tissue contents of acetylcholine and choline did not differ between experimental groups for either brain region. When hippocampal slices from the controls were incubated for 10 min with depolarizing concentrations of KCl, acetylcholine release increased and the tissue content decreased in a concentration‐dependent fashion; no net synthesis of acetylcholine occurred. In contrast, hippocampal slices from the choline‐injected animals maintained their tissue content in the presence of high concentrations of KCl, despite an increase in acetylcholine release that was similar in magnitude to that of the controls; positive net synthesis of acetylcholine resulted. Although the molar concentration of choline achieved in the incubation media at the end of the 10‐min period did not differ between groups, the mobilization of free choline from bound stores was significantly greater in hippocampal slices from the choline‐injected group than the controls. In addition, the synthesis of acetylcholine by hippocampal slices from the cholineinjected group was prevented by the presence of hemicholinium‐3 (1 μM) in the media. Acetylcholine synthesis and choline mobilization by striatal slices did not differ between groups. Results demonstrate that acute supplementation with choline provides precursor to support acetylcholine synthesis when the release of neurotransmitter is increased.
