Green Synthesis of Robust, Biocompatible Silver Nanoparticles Using Garlic Extract.
Green Synthesis of Robust, Biocompatible Silver Nanoparticles Using Garlic Extract.
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DOI：
10.1155/2012/730746
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发表时间：
2012
影响因子：
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通讯作者：
Gregory von White;Petra Kerscher;Ryan M Brown;Jacob D Morella;W. McAllister;D. Dean;C. Kitchens
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文献类型：
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作者：
Gregory von White;Petra Kerscher;Ryan M Brown;Jacob D Morella;W. McAllister;D. Dean;C. Kitchens
本文详细介绍了一种简便的方法，用于在环境温度/低温下合成稳定的单分散银纳米颗粒，其中大蒜提取物在纳米颗粒合成过程中用作银盐还原剂以及合成后稳定配体。改变合成条件提供了对颗粒尺寸、尺寸分布和颗粒形成动力学的控制。红外光谱、能量色散X射线化学分析和高效液相色谱表明，大蒜提取物中存在的碳水化合物是最有可能的纳米颗粒稳定化学物质。合成的银纳米颗粒还表现出生物医学应用的潜力，这是由于1）在生物介质中增强的稳定性，2）通过添加H2 O2而抗氧化，3）合成的容易性和可扩展性，以及4）缺乏合成所需的苛刻化学品。细胞毒性试验表明，在25 μg/ml的浓度下，血管平滑肌细胞和3 T3成纤维细胞的细胞增殖没有减少，证实了大蒜提取物制备的银纳米颗粒是未来实验和生物医学应用的理想候选物。
This paper details a facile approach for the synthesis of stable and monodisperse silver nanoparticles performed at ambient/low temperature where Allium sativum (garlic) extract functions as the silver salt reducing agent during nanoparticle synthesis as well as the post-synthesis stabilizing ligands. Varying the synthesis conditions provides control of particle size, size-distribution, and kinetics of particle formation. Infrared spectroscopy, energy dispersive x-ray chemical analysis, and high performance liquid chromatography indicated that the carbohydrates present in the garlic extract are the most likely nanoparticle stabilizing chemistry. The synthesized silver nanoparticles also demonstrate potential for biomeical applications, owing to the 1) enhanced stability in biological media, 2) resistance to oxidation by the addition of H2O2, 3) ease and scalability of synthesis, and 4) lack of harsh chemicals required for synthesis. Cytotoxicity assays indicated no decrease in cellular proliferation for vascular smooth muscle cells and 3T3 fibroblasts at a concentration of 25 μg/ml, confirming that garlic extract prepared silver nanoparticles are ideal candidates for future experimentation and implementation into biomedical applications.