A comparison of methodologies for the preparation of human epidermal-dermal composites
A comparison of methodologies for the preparation of human epidermal-dermal composites
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DOI：
10.1097/00000637-199710000-00010
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发表时间：
1997-10-01
影响因子：
1.5
通讯作者：
MacNeil, S
中科院分区：
文献类型：
--
作者：
Ghosh, MM;Boyce, S;MacNeil, S
本研究的目的是比较基于深层脱细胞真皮基质、表皮角质形成细胞和真皮成纤维细胞的人皮肤复合物的制备方法，目的是制备临床上有用的皮肤替代物。（甘油处理、环氧乙烷处理和γ辐射）、去除表皮的方法（氯化钠、磷酸盐缓冲盐水和分散酶）和用成纤维细胞和角质形成细胞接种复合物的方法。我们报告了在气液界面培养7天后可重复制备具有许多正常皮肤特征的复合材料的方案，这种复合材料可以基于用甘油或环氧乙烷预处理的异体真皮（尽管后者给出的结果不如甘油处理一致），通过在真皮的网状或乳头状表面上培养细胞可以实现成纤维细胞渗透到真皮中，然而，我们第一次报道了成纤维细胞从乳头状表面进入仅发生在角质形成细胞也存在时。
The purpose of this study was to compare methodologies for the preparation of human skin composites based on deepidermized acellular dermal matrix, epidermal keratinocytes, and dermal fibroblasts with the aim of preparing a clinically useful skin substitute, Dermal matrices were prepared from normal human skin and we compared methods of sterilization (glycerol treatment, ethylene oxide treatment, and gamma irradiation), methods of removing the epidermis (sodium chloride, phosphate buffered saline, and dispase), and methods of seeding the composites with fibroblasts and keratinocytes. We report protocols for reproducibly preparing composites that share many of the features of normal skin after 7 days culture at an air-liquid interface, Such composites can be based on allodermis pretreated with either glycerol or ethylene oxide (although the latter gave less consistent results than the glycerol treatment), Fibroblast penetration into the dermis could be achieved by culture of cells on the reticular or papillary surface of the dermis, However, we report for the first time that fibroblast entry from the papillary surface only occurred when keratinocytes were also present.