Radioreceptor assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting.
Radioreceptor assay for growth hormone in coho salmon (Oncorhynchus kisutch) and its application to the study of stunting.
复制标题
银大麻哈鱼（Oncorhynchus kisutch）生长激素的放射受体测定及其在发育迟缓研究中的应用。
DOI：
10.1002/jez.1402560308
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Bern,HA
中科院分区：
文献类型：
--
作者：
Gray,ES;Young,G;Bern,HA
用放射受体分析法分析了银鲑肝膜中天然鲑鱼生长激素（sGH）的结合位点。可置换（特异性）结合占添加的总放射性标记sGH的25%。结合取决于缓冲液pH和膜蛋白浓度，并且在15°C下24小时完成。特异性结合在肝膜中最大，并且在肌肉、卵巢、鳃、肾和脑中也检测到。Scatchard分析表明，一类肝脏结合位点对sGH具有特异性。在发育迟缓，异常海水鲑鱼血浆GEI水平升高和抑制生长，特异性结合的生长激素的肝膜低三倍thiin在正常的海水小鲑鱼。鲑鱼生长激素结合位点的浓度下降了60%，在矮化肝脏，而他们的亲和力生长激素是不变的。高血浆GH水平下调肝脏GH受体可能部分解释了发育迟缓中sGH结合率低的原因。
Binding sites for native chum salmon growth hormone (sGH) in coho salmon (Oncorhynchus kisutch) hepatic membranes were analyzed by radioreceptor assay. Displaceable (specific) binding represented up to 25% of total radiolabeled sGH added. Binding was dependent on buffer pH and membrane protein concentration, and was complete by 24 hours at 15°C. Specific binding was greatest in liver membranes, and was also detected in rr.uscle, ovary, gill, kidney, and brain. Scatchard analyses indicated a single class of hepatic binding sites that were specific for sGH. In stunts, abnormal seawater salmon with elevated plasma GEI levels and inhibited growth, specific binding of sGH to liver membranes was three times lower thiin in normal seawater smolts. The concentration of salmon GH binding sites was decreased in stunt livers by 60%, while their affinity for sGH was unchanged. Down‐regulation of hepatic GH receptors by high plasma GH levels may explain in part the low sGH binding in stunts.