Automated velocity programming for increased detection zone residence times in capillary electrophoresis.
Automated velocity programming for increased detection zone residence times in capillary electrophoresis.
复制标题
自动速度编程可增加毛细管电泳中检测区的停留时间。
DOI：
10.1021/ac00072a026
复制
发表时间：
1993
影响因子：
7.4
通讯作者：
Zare,RN
中科院分区：
文献类型：
--
作者：
Shear,JB;Colón,LA;Zare,RN
Frequently in chemical separations an analyte band yields a detectable signal that is too small to measure accurately. The lowest quantifiable analyte concentration is generally three to five times greater than the lowest detectable concentration. 12 Although the differencebetween the limit of quantitation (LOQ) and the limit of detection (LOD) is sometimes unavoidable—particularly when a large fraction of the analyte is chemically altered in the measurement process—it often results from an insufficient detection period. In these instances, all detectable species can be quantified without any modificationof the detectionhardware. We report the implementation of an ‘‘intelligent” detection system for capillary electrophoresis that responds rapidly to the detection of an analyte by directing a decrease in the separation field—and, hence, an increase in the measurement time—and demonstrate its ability to improve the LOQ almost to the LOD.For a wide range of column separation techniques (eg, HPLC, capillary chromatography, and capillary electrophoresis), analyte molecules commonly are probed briefly as they traverse either an on-line or a postcolumn flow cell and thus generatepeaks in a chromatogram or an electrophero-gram. This procedure is important for rapid and reproducible analyses, but it can severely limit the signal-noise ratio (SNR) of bands. In shot-noise-limited detectionprocedures, an
