Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger
Flow cytometric investigations of diploid and tetraploid plants and in vitro cultures of Datura stramonium and Hyoscyamus niger
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二倍体和四倍体植物以及曼陀罗和天仙子体外培养物的流式细胞术研究
作者：
J. Weber;V. Georgiev;A. Pavlov;T. Bley
植物离体系统是生产生物活性物质的重要来源。然而，次生代谢物的变化，以及可能由遗传不稳定性引起的低产量，使其工业实施复杂化。植物离体培养物的DNA谱分析可以为高产离体培养物的选择提供数据。用流式细胞术对二倍体、四倍体曼陀罗和黑山芋植株及其愈伤组织和毛状根线进行了分析。植株离体培养比外植体多经历几个内复制周期。生长因子诱导的愈伤组织的循环值最高，二倍体黑黑螺旋体为1.19。而在不添加生长因子的情况下，毛状根的内复制程度显著（二倍体黑螺旋体的循环值为0.88）。每株的5个毛状根系组和倍性水平在组内显示出一致的倍性模式。所研究的植物在体内和离体的倍性谱不同。我们首次报道了两种茄科植物的毛状根发生内复制。离体培养（毛状根和愈伤组织）的倍性谱似乎受基因组大小、所施用的生长因子和离体培养类型的影响。几种毛状根系的转化在倍性模式上没有差异。©2008国际细胞术进步学会
Plant in vitro systems are valuable sources for the production of biological active substances. However, changed profiles of secondary metabolites, and low, variable yields possibly caused by genetic instabilities complicate their industrial implementation. DNA profiling of plant in vitro cultures may provide data for the selection of highly producing in vitro cultures. Diploid and tetraploid Datura stramonium and Hyoscyamus niger plant as well as calli, and hairy root lines derived from them were analyzed by flow cytometry. Plant in vitro cultures undergo several cycles of endoreduplication more than the explants from which they were obtained. The highest cycle values were observed in calli (e.g. 1.19 for diploid H. niger) possibly induced by the growth factors. However, hairy roots cultivated without growth factor exhibited significant degrees of endoreduplication (cycle value 0.88 for diploid H. niger). Sets of five hairy root lines from each plant and ploidy level showed consistent within‐set ploidy patterns. The ploidy profiles of investigated plant in vitro and in vivo differ. For the first time we report that hairy roots of two Solanaceae species undergo endoreduplication. Theploidy profiles of in vitro cultures (hairy roots and calli) seem to be influenced by the genome size, the growth factors applied, and the type of in vitro culture. The transformation of several hairy root lines showed no differences in the ploidy patterns. © 2008 International Society for Advancement of Cytometry