Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis
Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis
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DOI：
10.1093/dnares/7.1.9
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发表时间：
2000-02-28
期刊：
影响因子：
4.1
通讯作者：
Hasebe, M
中科院分区：
文献类型：
--
作者：
Nishiyama, T;Hiwatashi, Y;Hasebe, M
小立小苔因其简单的形体结构，已被广泛应用于许多领域。基因定位的便捷性，以及其他原因。虽然报道了许多突变体，但没有方法分离相应的基因。利用穿梭诱变技术，建立了一套用于芽殖酵母的基因标记和诱捕系统。在5264个标记系中，获得203个发育或形态表型改变的突变体。在4757个基因诱捕系中，有129个在部分组织中检测到β -葡萄糖醛酸酶（GUS）活性。尽管Southern分析在许多标记系中检测到一个标签的多个拷贝，但根据PCR分析，大多数拷贝可能整合在同一个位点上。
The moss, Physcomitrella patens has been used as a useful material in many fields, because of its simple body plan., ease of gene targeting, and other reasons. Although many mutants have been reported, no method to isolate the corresponding genes was reported. We developed a gene tagging and gene-trap system in P. patens by using the shuttle mutagenesis technique, which has been used in the budding yeast. In 5264 tagged lines, 203 mutants with altered developmental or morphological phenotypes were obtained. In 129 of 4757 gene-trap lines, beta-glucuronidase (GUS) activity was detected in some tissue. Although multiple copies of a tag were detected in many tagged lines by Southern analyses, most copies are likely integrated at the same locus according to PCR analyses.