Changes in root architecture and endogenous hormone levels in two Malus rootstocks under alkali stress
Changes in root architecture and endogenous hormone levels in two Malus rootstocks under alkali stress
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DOI：
10.1016/j.scienta.2017.09.015
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发表时间：
2018-05
影响因子：
4.3
通讯作者：
T. Wen;L. Dong;Lei Wang;F. Ma;Y. Zou;Cuiying Li
中科院分区：
文献类型：
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作者：
T. Wen;L. Dong;Lei Wang;F. Ma;Y. Zou;Cuiying Li
土壤pH值过高是中国北方地区严重的农业问题，影响苹果的新陈代谢，特别是根系发育。为了解释苹果植株对碱胁迫（非胁迫对照pH6.0 ± 0.1，碱胁迫处理pH9.0 ± 0.1）的生理反应，采用水培方法研究了两种不同耐碱性砧木的表现。这些试验包括两种碱性盐（Na 2CO 3：NaHCO 3; 1：1，v：v），旨在模拟中国北方的土壤条件。对于这两个砧木，鲜重和干重，株高，根长，根活性值下降，而在根中的相对电解质渗漏增加时，与各自的控制相比，在应力处理。然而，这一反应的幅度小得多，在碱敏感M. rockii的耐碱性Malusprunifoliathan。碱胁迫对内源激素的影响也在胁迫处理的砧木和对照之间存在显著差异。碱胁迫处理15天后，M.与对照相比，二氢玉米素核苷（DHZR）和茉莉酸（JA）含量降低，吲哚乙酸（IAA）、脱落酸（阿坝）、赤霉素（GA 3）和玉米素核苷（ZR）含量增加，其中阿坝、ZR和JA含量与对照差异显著。至于M。结果表明，在AS胁迫下，根中阿坝、IAA、JA和DHZR含量降低，而GA 3和ZR含量升高，其中阿坝、IAA、JA和ZR含量与对照差异显著。碱胁迫下两种苹果砧木幼苗根系中阿坝和IAA含量差异显著。特别是根中的IAA含量，M处理比对照提高了30.0%。在M. rockii。碱胁迫处理15 d后，岩松根构型受碱胁迫的影响也比岩松严重。桃叶。与对照相比，M. rockii几乎是M的两倍。桃叶。特别是根尖数，M. rockii是M的17.6倍。桃叶。根构型的这些差异可以用激素活性的变化来解释，特别是IAA。
High soil pH is a serious agricultural problem in northern China, where it affects apple metabolism, specifically root development. To explain the physiological responses of apple plants to alkali stress (non-stressed control, pH 6.0 ± 0.1, versus alkali stress treatment, pH 9.0 ± 0.1), we used hydroponics culturing to study the performance of two rootstocks with contrasting degrees of alkali tolerance. These trials included two alkaline salts (Na2CO3:NaHCO3; 1:1, v:v) that were intended to simulate soil conditions in northern China. For both rootstocks, values for fresh and dry weights, plant heights, root lengths, and root activity were decreased in response to stress treatment while relative electrolyte leakage in the roots was increased when compared with their respective controls. However, the amplitude of this response was much smaller in the alkali-tolerantMalus prunifoliathan in the alkali-sensitiveM.rockii. The effects of alkali stress on endogenous hormones also varied significantly between stress-treated rootstocks and their controls. After 15 days of alkali stress treatment, forM. prunifolia(AT), compared with the control, the contents of dihydrozeatin riboside (DHZR) and jasmonic acid (JA) were decreased while the contents of indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellin (GA3) and zeatin riboside (ZR) were increased in the roots, among which the contents of ABA, ZR and JA in the roots were significantly different from their controls. As forM. rockii(AS), the contents of ABA, IAA, JA and DHZR were decreased while the content of GA3and ZR were increased in the roots, among which ABA, IAA, JA and ZR in the roots were significantly different from the controls. However, there were significant differences in the contents of ABA and IAA in the roots between two apple rootstocks seedlings under alkali stress. Especially for the IAA levels in the roots, compared with their controls, was increased by 30.0% inM. prunifoliabut decreased by 17.8% inM. rockii. After 15 days of alkali stress treatment, the root architecture ofM.rockiiwas also more seriously influenced by alkali stress compared withM. prunifolia. Compared with their controls, the descent range of each index of the root architecture ofM. rockiiwas almost twice as high as that ofM. prunifolia. Especially for the number of root tips, the decrease amplitude ofM. rockiiwas 17.6 times greater than that ofM. prunifolia. These differences in root architecture might be explained by changes in the activity of hormones, especially for IAA.