Enzyme immunoassays for 15-F2T isoprostane-M, an urinary biomarker for oxidant stress.
Enzyme immunoassays for 15-F2T isoprostane-M, an urinary biomarker for oxidant stress.
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15-F2T 异前列烷-M（氧化应激的尿液生物标志物）的酶免疫分析。
DOI：
10.1007/978-1-4615-0193-0_82
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发表时间：
2002
影响因子：
--
通讯作者：
Callewaert,DenisM
中科院分区：
文献类型：
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作者：
Sasaki,DianeM;Yuan,Ying;Gikas,Katherine;Kanai,Kazuo;Taber,Douglass;Morrow,JasonD;Roberts2nd,LJackson;Callewaert,DenisM
异前列烷测定具有用作人类氧化应激分子生物标志物的巨大潜力（Morrow 等人，1999 年；Lawson 等人，1999 年）。最近的研究表明：(a) 大量源自全身氧化应激的 F2 异前列烷在排泄前被代谢，(b) 大量游离 F2 异前列烷由肾脏产生，因此尿液中检测到的游离异前列烷可能无法准确反映全身氧化应激，以及 (c) 2,3-dinor-5,6-diHydro-15-F2tIsoprostane (F2-IsoP-M， IsoP-M) 是全身性 8-iso-PG F2Œ 的一种主要尿液代谢物，最近已被鉴定出来（图 1）。因此，尿液 F2-IsoP-M 可能被证明是全身氧化应激的极好标志物。我们的目标是开发一种灵敏且特异的 F2-IsoP-M 免疫测定方法，为人体氧化应激的常规定量评估提供一种简单的非侵入性方法。
Isoprostane assays have significant potential for use as molecular biomarkers of human oxidant stress (Morrow et al. 1999, Lawson et al. 1999). Recent studies indicate that: (a) significant amounts of F2 isoprostanes derived from systemic oxidant stress are metabolized before excretion, (b) a significant quantity of free F2 isoprostanes are produced by the kidney, so free isoprostanes detected in urine may not accurately reflect systemic oxidant stress and (c) 2,3-dinor-5,6-dihydro-15-F2tIsoprostane (F2-IsoP-M, IsoP-M), a major urinary metabolite of systemic 8-iso-PG F2Œ, has recently been identified (Figure 1). Urinary F2-IsoP-M may, therefore, prove to be an excellent marker for systemic oxidant stress. Our goal is to develop a sensitive and specific immunoassay for F2-IsoP-M, which should provide a simple non-invasive method for routine quantitative assessment of human oxidant stress.