Estrogenic Effects of the Herbal formula, Menoprogen, in Ovariectomized Rats
Estrogenic Effects of the Herbal formula, Menoprogen, in Ovariectomized Rats
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DOI：
10.1248/bpb.33.455
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发表时间：
2010-03-01
影响因子：
2
通讯作者：
Hattori, Masao
中科院分区：
文献类型：
--
作者：
Ma, Hong;Chung, Mi Hwa;Hattori, Masao
尽管绝经后妇女的健康风险，激素替代疗法的适应症和理想的候选人仍然不清楚。本研究采用去卵巢大鼠，研究了治疗更年期综合征的中药复方Menoprogen（MPG）1的安全性和有效性。每日口服MPG（1000 mg/kg，体重）2周，显著恢复子宫和肾上腺萎缩，并恢复血清雌二醇。雌酮和孕激素水平降低大鼠双侧卵巢切除术。然而.酵母双杂交和核受体辅因子分析显示MPG不抑制雌激素受体α（ER α）和P，免疫组织化学染色显示MPG不刺激子宫内ER α、孕酮受体、c-jun和c-fos的蛋白表达。MPG在体内无副作用。这些结果表明，MPG将是有用的治疗妇女绝经前和绝经后综合征。
Despite the health risks for postmenopausal women, the indications and ideal candidates for hormone replacement therapy remain unclear. The present study used ovariectomized rats to examine the safety and effects of the Chinese herbal formula Menoprogen (MPG)1 Which is prescribed for menopausal syndrome. Daily oral MPG (1000 mg/kg, body weight) for 2 weeks significantly recovered uterine and adrenal gland atrophy and restored serum estradiol. estrone and progesterone levels that were decreased in rats by bilateral ovariectomy. However. yeast two-hybrid and nuclear receptor cofactor assays showed that MPG did not hind estrogen receptors alpha (ER alpha) and P, and immunohistochemical staining revealed that unlike 17 beta-estradiol, M PC did not stimulate the protein expression of ER alpha, progesterone receptor, c-jun and c-fos in (lie uterus. No side effects of MPG were confirmed in vivo. These findings suggest that MPG would he useful for treating women with premenopausal and postmenopausal syndromes.