Zipper-like magnetic molecularly imprinted microspheres for on/off-switchable recognition and extraction of 17 beta-estradiol from food samples
Zipper-like magnetic molecularly imprinted microspheres for on/off-switchable recognition and extraction of 17 beta-estradiol from food samples
复制标题
拉链状磁性分子印迹微球用于可开/关切换地识别和从食品样品中提取 17 β-雌二醇
DOI：
10.1016/j.foodchem.2018.04.034
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发表时间：
2018
期刊：
影响因子：
8.8
通讯作者：
Li Yanbin
中科院分区：
文献类型：
--
作者：
Zhu Wenting;Peng Hailong;Luo Mei;Yu Ningxiang;Xiong Hua;Wang Ronghui;Li Yanbin
以丙烯酰胺（AAm）和2-丙烯酰胺-2-甲基丙磺酸（AMPS）为功能单体，构建了具有开关功能的磁性分子印迹微球（SM-MIMs），用于17β-雌二醇（17β-E2）的识别和萃取。印迹聚合物聚丙烯酸酰胺（PAAm）和聚丙烯酸单酰胺（PAMPS）之间的相互作用具有温度开关特性，在较高的温度（如30 °C）下表现出解离作用，有助于17β-E2进入印迹位点，从而提高了印迹聚合物的结合能力。相反，PAAm和PAMPS之间的聚合物复合物在较低温度（如20 °C）下形成并阻断17β-E2进入印迹位点。将SM-MIMs作为分散固相萃取（SPE）吸附剂用于食品样品中17β-E2的前处理和HPLC检测，获得了较低的检出限（2.52 μg L−1）和定量（10.76 μg L−1），且回收率较高。因此，SM-MIMs具有可开关识别、环境友好洗脱和高效分离等优点，有望成为食品样品中17β-E2预处理的理想吸附剂。
Zipper-like on/off-switchable and magnetic molecularly imprinted microspheres (SM-MIMs) were constructed using acrylamide (AAm) and 2-acrylamide-2-methyl propanesulfonic acid (AMPS) as functional monomers for 17β-estradiol (17β-E2) recognition and extraction. The imprinted polymer interactions between poly(AAm) (PAAm) and poly(AMPS) (PAMPS) with on/off-switchable property to temperature, exhibited dissociation at relatively higher temperatures (such as 30 °C) and helped 17β-E2enter into imprinted sites, leading to higher binding capability. Conversely, the interpolymer complexes between PAAm and PAMPS formed and blocked 17β-E2access to imprinted sites at lower temperature (such as 20 °C). SM-MIMs were used as dispersive solid phase extraction (SPE) adsorbent with HPLC for 17β-E2pretreatment and detection in food samples, and low limit detection (2.52 µg L−1) and quantification (10.76 µg L−1) with higher recovery were obtained. Therefore, SM-MIMs may be a promising adsorbent for 17β-E2pretreatment in food samples owing to its advantages of on/off-switchable recognition, eco-friendly elution, and efficient separation.