Proteomic analysis of cucumber seedling roots subjected to salt stress
Proteomic analysis of cucumber seedling roots subjected to salt stress
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盐胁迫下黄瓜幼苗根部的蛋白质组学分析
DOI：
10.1016/j.phytochem.2010.05.020
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发表时间：
2010-09-01
期刊：
影响因子：
3.8
通讯作者：
Li, Juan
中科院分区：
文献类型：
--
作者：
Du, Chang-Xia;Fan, Huai-Fu;Li, Juan
为了解盐胁迫下植物代谢的变化，研究了黄瓜（Cucumis sativus L. cv.）的生理生化反应。研究了金春2号幼苗对盐胁迫的反应。NaCl处理显著降低了黄瓜幼苗根系干重和鲜重；Na+和Cl-升高，K+和K+/Na+比值降低。为了确定盐胁迫信号的组成，我们比较了对照和nacl处理的根的高分辨率二维凝胶电泳（2-DE）蛋白谱，发现34个蛋白斑点的强度发生了变化。利用基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF-MS）和液相色谱电喷雾串联质谱法（LC-ESI-MS/MS）对29个（盐胁迫诱导的上调蛋白点21个，下调蛋白点8个）进行了鉴定。大多数蛋白质具有与代谢、能量和运输有关的功能，并参与调节反应和抵御压力。采用基于肽序列的半定量逆转录聚合酶链反应（PCR）方法比较了10个候选蛋白的转录和蛋白质积累模式。其中，8种诱导转录物积累模式与诱导蛋白质积累模式一致。因此，植物蛋白质组对NaCl胁迫的反应可能是复杂的，所鉴定的蛋白质可能在调控NaCl胁迫后的适应活动中发挥重要作用，以促进离子稳态。(C) 2010 Elsevier Ltd.版权所有。
To understand metabolic modifications in plants under salt stress, the physiological and biochemical responses of cucumber (Cucumis sativus L. cv. Jinchun No. 2) seedlings to salt stress was investigated. The dry weight and fresh weight of cucumber seedling roots were significantly reduced by treatment with NaCl; Na+ and Cl- were increased, while K+ and K+/Na+ ratio were decreased. To identify components of salt stress signaling, we compared the high resolution two-dimensional gel electrophoresis (2-DE) protein profiles of control and NaCl-treated roots, and the intensity of 34 protein spots varied. Of these spots, the identities of 29 (21 up-regulated and 8 down-regulated protein spots induced after salt stress) were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography electro-spray ionization tandem mass spectrometry (LC-ESI-MS/MS). The majority of the proteins had functions related to metabolism, energy and transport, and are involved in regulating reactions and defending against stress. A semi-quantitative reverse transcriptional-polymerase chain reaction (PCR) approach based on peptide sequences was used to compare transcript and protein accumulation patterns for 10 candidate proteins. Of these proteins, 8 patterns of induced transcript accumulation were consistent with those of induced protein accumulation. It is therefore likely that the response of the plant's proteome to NaCl stress is complex, and that the identified proteins may play an important role in regulating adaptation activities following exposure to NaCl stress in order to facilitate ion homeostasis. (C) 2010 Elsevier Ltd. All rights reserved.