Effects of gender, body composition, and menopause on plasma concentrations of leptin
Effects of gender, body composition, and menopause on plasma concentrations of leptin
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DOI：
10.1210/jc.81.9.3424
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发表时间：
1996-09-01
影响因子：
5.8
通讯作者：
Leibel, RL
中科院分区：
文献类型：
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作者：
Rosenbaum, M;Nicolson, M;Leibel, RL
瘦素的循环浓度（[瘦素]）与体重指数和体脂百分比直接相关，因此可能构成一个调节体脂的系统的传入支。我们检验了以下假设：1）血浆[瘦素]与绝对脂肪量的直接相关性比与体脂百分比本身更强；2）脂肪量与[瘦素]之间的关系受性别和绝经状态的显著影响。在67名受试者（26名男性，20名绝经前女性，21名绝经后女性；43名从未肥胖者，24名肥胖者）处于正常体重时，检测其吸收后状态的[瘦素]。通过水下称重法测定身体成分，通过双抗体酶联免疫吸附测定法测定[瘦素]。在男性和绝经前女性受试者中，通过锇固定法进行皮下脂肪组织抽吸以测定脂肪细胞体积，并进行3小时口服葡萄糖耐量试验。在正常体重时，（[瘦素]与绝对脂肪量的相关性比与体重指数（BMI）或体脂百分比更好。BMI和体脂百分比本身并不能解释[瘦素]中超出可归因于脂肪量（FM）的任何变异。肥胖和从未肥胖的受试者之间，脂肪量与[瘦素]相关的回归方程没有显著差异。[瘦素]和空腹血清胰岛素浓度仅在男性中显著相关。绝经前和绝经后女性的[瘦素]显著高于男性，即使对[瘦素]进行校正后也是如此。绝经后女性的[瘦素]经脂肪量校正后显著下降，但这种下降不足以解释瘦素与脂肪量关系中显著的性别差异。这种性别差异显然也部分是由于循环雄激素对[瘦素]的抑制作用。
Circulating concentrations of leptin ([leptin]) vary directly with body mass index and percentage body fat, and may thus constitute an afferent limb of a system regulating body fatness. We tested the hypotheses that: 1). Plasma [leptin] vary more directly with absolute fat mass than with fractional body fatness per se; and 2). The relationship between fat mass and [leptin] is significantly affected by gender and by menopausal status. [leptin] in the post-absorptive state was examined in 67 subjects (26 male, 20 premenopausal female, 21 post-menopausal females; 43 never-obese, 24 obese) at usual body weight. Body composition was determined by hydrodensitometry, and [leptin] wase determined by a double antibody ELISA assay. In male and pre-menopausal female subjects, subcutaneous adipose tissue aspirations were performed for determination of adipocyte volume by the osmium fixation method, and a 3 hour oral glucose tolerance tests was performed. At usual body weight, ([leptin] was better correlated with absolute fat mass than with body mass index (BMI) or percentage body fat. BMI and % body fat did not account for any of the variance in [leptin] beyond that attributable to FM, per se. The regression equations relating FM to [leptin] did not differ significantly between obese and never-obese subjects. [leptin] and fasting serum insulin concentrations were significantly correlated in males only. [Leptin] was significantly higher in pre- and post-menopausal females compared to males, even when [leptin] was corrected for [leptin], corrected for FM, declines significantly in women post-menopause, this decline is not sufficient to account for the striking sexual dimorphism in the relationship of leptin to fat mass. This sexual dimorphism is apparently also due, in part, to a suppressive effect of circulating androgens on [leptin].